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Dechlorane Plus (DP) is found widely in the environment. It is important to study DP enrichment and biomagnification in terrestrial ecosystems to improve our understanding of the possible effects of DP on the environment and human health. A total of 90 samples, including plant and animal tissues, were collected from Xilingol Prairie in Inner Mongolia, China. The DP concentrations in different species were assessed, and transmission of DP through food webs containing ectotherms and endotherms was assessed. The compound was detected in the biotic samples (plant; range 0.133–0.422 ng/g dry wt), in animal muscle (range: not dected–5.70 ng/g lipid wt), and in animal hair (range: not dected–2.03 ng/g dry wt), indicating that DP is present in remote environments such as Xilingol Prairie. These findings suggest that DP can undergo long‐distance transport in the environment. Biomagnification factors (ectotherms: range 0.146–88.0, endotherms: range 0.866–17.2) and anti‐DP/total DP concentration ratios (fanti values of 0.412–0.787) for the prairie animals were calculated. Ectotherms were found to selectively enrich syn‐DP, and stereoselective enrichment increased moving up the food web. Lower‐trophic‐level endotherms strongly stereoselectively enriched syn‐DP, and higher‐trophic‐level endotherms stereoselectively enriched anti‐DP. Environ Toxicol Chem 2021;40:413–421. © 2020 SETAC

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