This study attempts to elucidate the emission sources and mechanisms of nitrous oxide (N2O) during simultaneous nitrification and denitrification (SND) process under oxygen-limiting condition. The results indicated that N2O emitted during low-oxygen SND process was 0.8±0.1 mgN/gMLSS, accounting for 7.7% of the nitrogen input. This was much higher than the reported results from conventional nitrification and denitrification processes. Batch experiments revealed that nitrifier denitrification was attributed as the dominant source of N2O production. This could be well explained by the change of ammonia-oxidizing bacteria (AOB) community caused by the low-oxygen condition. It was observed that during the low-oxygen SND process, AOB species capable of denitrification, i.e., Nitrosomonas europaea and Nitrosomonas-like, were enriched whilst the composition of denitrifiers was only slightly affected. N2O emission by heterotrophic denitrification was considered to be limited by the presence of oxygen and unavailability of carbon source.
Three laboratory scale anaerobic-aerobic (low-oxygen) SBRs (R1, R2 and R3) were conducted at different influent phosphorus concentration to evaluate the impacts of phosphorus load on nutrients removal and nitrous oxide (N₂O) emission during low-oxygen simultaneous nitrification and denitrification (SND) process. The results showed that TP and TN removals were enhanced simultaneously with the increase in phosphorus load. It was mainly caused by the enrichment of polyphosphate accumulating organisms (PAOs) under high phosphorus load and low COD/P ratio (<50), which could use nitrate/nitrite as electron acceptors to take up the phosphorus. N₂O emission was reduced with increasing phosphorus load. N₂O-N emission amount per cycle of R3 was 24.1% lower than that of R1. It was due to the decrease of N₂O yield by heterotrophic denitrification. When the phosphorus load increased from R1 to R3, heterotrophic denitrification (D) ranged from 42.6% to 36.6% of the N₂O yield.
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