A Gram-stain-negative, rod-shaped, motile bacterial strain, designated 3-5-3 T , was isolated from maize-cultivated soil artificially contaminated with cadmium, in Nanyang, Henan Province, China. Strain 3-5-3 T was oxidase-and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-5-3 T was affiliated with the genus Paenibacillus and most closely related to Paenibacillus anaericanus MH2 T (96.5 % similarity). The average nucleotide identity and digital DNA-DNA hybridization values between 3-5-3 T and the closely related species ranged 69.4-84.5 % and 18.1-18.4 %. The genomic G+C content was 53.8 mol%. Anteiso-C 15 : 0 was the major fatty acid and MK-7 was the only menaquinone. The diamino acid in the cell-wall peptidoglycan contains meso-diaminopimelic acid. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, two unidentified glycolipids, two unidentified aminolipids, one unidentified phospholipid, one unidentified phosphoaminolipid and six unidentified lipids. On the basis of the results obtained in this study, strain 3-5-3 T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacilluszei soli sp. nov. is proposed. The type strain is 3-5-3 T (=CGMCC 1.13686 T =KCTC 33998 T).
A Gram-stain-positive, rod-shaped, motile bacterial strain, designated 3-2-2 T , was isolated from field topsoil collected from a western suburb of Nanyang city, Henan province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-2-2 T was a member of the genus Bacillus and most closely related to Bacillus fortis R-6514 T (98.9 % similarity), Bacillus terrae RA9 T (98.0 %) and Bacillus fordii R-7190 T (97.7 %). A draft genome sequence determined for strain 3-2-2 T revealed a DNA G+C content of 42.2 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between 3-2-2 T and the closely related Bacillus species ranged 79.4-84.2 % and 23.4-24.6 %. The major fatty acids of strain 3-2-2 T were iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 14 : 0 and iso-C 16 : 0. The major isoprenoid quinone was MK-7. meso-Diaminopimelic acid was detected in the peptidoglycan. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. The results of phylogenetic analyses, in silico genomic comparisons, and chemotaxonomic and phenotypic analyses clearly indicated that strain 3-2-2 T represents a novel species within the genus Bacillus, for which the name Bacillus acidinfaciens sp. nov. is proposed. The type strain is 3-2-2 T (=CGMCC 1.13685 T =LMG 30839 T).
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