Circular RNAs (circRNAs) are a class of powerful regulators of gene expression. This study aimed to determine whether circTRRAP (hsa_circ_0081241) was implicated in the cardioprotective effects of salvianolic acid B (Sal B) against myocardial ischemia/reperfusion (I/R) injury and its associated mechanism.Cell viability was analyzed using Cell Counting Kit-8 (CCK-8), and flow cytometry was conducted to evaluate cell cycle progression and cell apoptosis. The leakage of lactic dehydrogenase (LDH), production of malondialdehyde (MDA), and activity of superoxide dismutase (SOD) were measured using their corresponding commercial kits to analyze cell death and oxidative stress.I/R treatment suppressed viability and cell cycle progression and induced the apoptosis and oxidative stress of AC16 cardiomyocytes, whereas Sal B protected AC16 cardiomyocytes against I/R injury. I/R upregulated circTRRAP expression, whereas Sal B dose-dependently reduced the circTRRAP level in AC16 cardiomyocytes. The protective effects of Sal B in I/R-induced AC16 cardiomyocytes were overturned by the overexpression of circTRRAP. CircTRRAP negatively regulated miR-214-3p expression by binding to it in AC16 cardiomyocytes. The circTRRAP overexpression-mediated effects were reversed by the addition of miR-214-3p mimics in AC16 cardiomyocytes. MiR-214-3p targeted the 3'-untranslated region (3'UTR) of SOX6, and SOX6 was regulated by the circTRRAP/miR-214-3p axis in AC16 cardiomyocytes. SOX6 knockdown overturned the circTRRAP overexpression-induced effects in AC16 cardiomyocytes.In conclusion, the silence of circTRRAP was implicated in Sal B-mediated cardioprotective effects against I/R injury by regulating the miR-214-3p/SOX6 axis.
Objective. To explore the application effect of new material after surface modification of zirconia ceramics and patient evaluation. Methods. A total of 60 patients with tooth defect treated in our hospital from April 2020 to April 2021 were selected as the study subjects and randomly divided into the control group and experimental group, with 30 cases each. The patients in the control group were treated with glass-ceramics, and those in the experimental group received LiSi surface treatment, so as to compare the application effect and patients’ evaluation between the two groups. Results. Between the two groups, no obvious differences in surface loss, adhesive strength, and transmittance at 3 months, 6 months, and 1 year were not observed (
P
>
0.05
); and after intervention, the score on dental aesthetics, hardness value, and occlusal force were obviously higher in the experimental group than in the control group (
P
<
0.001
). Conclusion. The new material enables forming an acid etchable coating on the zirconia surface, increases the adhesive strength, and achieves an aesthetic degree that is welcomed by the patients; meanwhile, after grinding, the edge is defect free and the tightness is higher. Further research will help to establish a better solution for patients.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.