MicroRNAs (miRNAs) play an essential role in cancer therapy,
but
the disadvantages of its poor inherent stability, rapid clearance,
and low delivery efficiency affect the therapeutic efficiency. Loading
miRNAs by nanoformulations can improve their bioavailability and enhance
therapeutic efficiency, which is an effective miRNA delivery strategy.
In this study, we synthesized layered double hydroxides (LDH), which
are widely used as carriers of drugs or genes due to the characteristics
of good biocompatibility, high loading capacity, and pH sensitivity.
We loaded the suppressor oncogene miR-30a on LDH nanomaterials (LDH@miR-30a)
and determined the mass ratio of miRNA binding to LDH by agarose gel
electrophoresis. LDH@miR-30a was able to escape the lysosomal pathway
and was successfully phagocytosed by breast cancer SKBR3 cells and
remained detectable in the cells after 24 h of co-incubation. In vitro
experiments showed that LDH@miR-30a-treated SKBR3 cells showed decreased
proliferation and cell cycle arrest in the G0/G1 phase and LDH@miR-30a
was able to regulate the epithelial–mesenchymal transition
(EMT) process and inhibit cell migration and invasion by targeting
SNAI1. Meanwhile, in vivo experiments showed that nude mice treated
with LDH@miR-30a showed a significant reduction in their solid tumors
and no significant impairment of vital organs was observed. In conclusion,
LDH@miR-30a is an effective drug delivery system for the treatment
of breast cancer.
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