We report a general method for light-assisted control of interactions of PDZ domain binding motifs with their cognate domains by the incorporation of a photolabile caging group onto the essential C-terminal carboxylate binding determinant of the motif. The strategy was implemented and validated for both simple monovalent and biomimetic divalent ligands, which have recently been established as powerful tools for acute perturbation of native PDZ domain-dependent interactions in live cells.
The kinetic isotope effect in the Cr(VI) oxidation of commercially available 2-propanol-d 8 or methanol-d 4 is readily demonstrated using 4 mL reaction vials projected with a document camera. For the case of 2-propanol, the demonstration reveals how a rate-determining step can be identified with an isotopically labeled compound, providing students with an example of how mechanistic evidence is experimentally determined. The 2-propanol oxidation demonstration has been documented in a YouTube video, which also discusses the relevant chemistry.
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