Variation in the spatial distribution of leaf chlorophyll content associated with the progression of the phenological cycle of crops may occur in cultivated areas as a result of the variability of environmental conditions and of the intrinsic properties of the plants. The objective of the present study was to model the trend in variation and assess the temporal stability of index of chlorophyll a, b, and total chlorophyll (Chla, Chlb, and Chlt, respectively), and to characterize the spatial distribution of Chlt index in grapevine cv. 'Chardonnay'. The assessments consisted of in situ measurements made with a portable meter in a commercial vineyard located in the municipality of Espírito Santo do Pinhal, state of São Paulo, Brazil, in the period between flowering and fruit maturation. Descriptive statistics were applied to the indices and regression models were fitted to ascertain the relationship of their mean variation with time. The temporal stability of Chlt index was estimated using Spearman's rank correlation analysis and thematic maps were created using geostatistical analysis and spatial estimation by ordinary kriging. The Chlb and Chlt indices were non-linearly associated with cycle progression and their decrease after the start of maturation was estimated. The temporal stability of the Chlt index was low and variation in its spatial distribution was observed over the assessed period.