Background Gut microbiota mainly function in the biotransformation of primary ginsenosides into bioactive metabolites. Herein, we investigated the effects of three prebiotic fibers by targeting gut microbiota on the metabolism of ginsenoside Rb1 in vivo . Methods Sprague Dawley rats were administered with ginsenoside Rb1 after a two-week prebiotic intervention of fructooligosaccharide, galactooligosaccharide, and fibersol-2, respectively. Pharmacokinetic analysis of ginsenoside Rb1 and its metabolites was performed, whilst the microbial composition and metabolic function of gut microbiota were examined by 16S rRNA gene amplicon and metagenomic shotgun sequencing. Results The results showed that peak plasma concentration and area under concentration time curve of ginsenoside Rb1 and its intermediate metabolites, ginsenoside Rd, F2, and compound K (CK), in the prebiotic intervention groups were increased at various degrees compared with those in the control group. Gut microbiota dramatically responded to the prebiotic treatment at both taxonomical and functional levels. The abundance of Prevotella , which possesses potential function to hydrolyze ginsenoside Rb1 into CK, was significantly elevated in the three prebiotic groups (P < 0.05). The gut metagenomic analysis also revealed the functional gene enrichment for terpenoid/polyketide metabolism, glycolysis, gluconeogenesis, propanoate metabolism, etc. Conclusion These findings imply that prebiotics may selectively promote the proliferation of certain bacterial stains with glycoside hydrolysis capacity, thereby, subsequently improving the biotransformation and bioavailability of primary ginsenosides in vivo .
The environment in urban residential areas is the main field of daily activity for the elderly. Environmental renewal has played a significant role in improving residents’ quality of life and promoting physical and mental health. However, there is a general tendency that more attention has been focused on the environment during environmental renewal but not the residents. There is a continued lack of discussion on the emotional status of the elderly and its impact on outdoor activities. Based on the investigation of four types of typical residential environments in the Dalian residential area, a hierarchical linear model (HLM) has been constructed to reveal the influence of the emotional status of elderly persons on their activity characteristics and the selection of environmental quality factors. The results show that the distribution of older people with different emotional statuses varies among different residential types. The proportion of positive emotion is relatively high in the flat land in rows category, and the activity characteristics are significantly different under different emotional statuses. Among the four kinds of residential environments, which are (flat land in rows, flat land enclosed, slope, and mountainous) the environmental quality factors that have the highest impact on the emotional status of the elderly are greening quality (0.395), acoustic environment (0.167), environmental cleanliness (0.269), and greening quality (0.230), respectively. In the mountainous type, the impact of environmental quality factors on the emotional status of the elderly is the highest (39.7%), and the impact contributions of the other three environmental types are 23.3%, 8.9% and 20.1%, respectively. These research results provide helpful guidance for the scientific community about practical implementation of residential environmental renewal for the elderly.
To investigate the effect of Chinese medicine raw materials and production technology on quality consistency of Chinese patent medicines with Gegen Qinlian decoction as an example, and establish a suitable method for the quality consistency control of Chinese patent medicines. The results showed that the effect of production technology on the quality consistency was generally not more than 5%, while the effect of raw materials was even more than 30%, indicating that the effect of raw materials was much greater than that of the production technology. In this study, blend technology was used to improve the quality consistency of raw materials. As a result, the difference between the product produced by raw materials and reference groups was less than 5%, thus increasing the quality consistence of finished products. The results showed that under the current circumstances, the main factor affecting the quality consistency of Chinese patent medicines was raw materials, so we shall pay more attention to the quality of Chinese medicine's raw materials. Finally, a blend technology can improve the quality consistency of Chinese patent medicines.
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