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These studies and others support a mechanism whereby an exchange-labile Cou(phen)x complex is rapidly and reversibly chelated by SF,. The Con(phen)x-enzyme complex is oxidized in situ by [Com(phen)2C03]+ to yield an exchange-inert ConI(phen)x-SF, complex with no ATPase activity. Inactivations of SF, which result from Con(phen)x in situ oxidation by either H202 or 02 are not reversed by reducing agents, despite the removal of bound cobalt. These data indicate in situ oxidation of CoII(phen)x by the single electron-accepting oxidant [ConI(phen)2C03]+ results in a very mild incorporation of Co(III) into SF,. This approach may have general application for the incorporation of Co(III) into proteins.agents which convert the exchange-inert cobalt(III) back to exchange labile Co(II).

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