Understanding humoral responses to SARS-CoV-2 is critical for improving diagnostics, therapeutics, and vaccines. Deep serological profiling of 232 COVID-19 patients and 190 pre-COVID-19 era controls using VirScan revealed over 800 epitopes in the SARS-CoV-2 proteome, including 10 epitopes likely recognized by neutralizing antibodies. Pre-existing antibodies in controls recognized SARS-CoV-2 ORF1, while only COVID-19 patients primarily recognized spike and nucleoprotein. A machine learning model trained on VirScan data predicted SARS-CoV-2 exposure history with 99% sensitivity and 98% specificity; a rapid Luminex-based diagnostic was developed from the most discriminatory SARS-CoV-2 peptides. Individuals with more severe COVID-19 exhibited stronger and broader SARS-CoV-2 responses, weaker antibody responses to prior infections, and higher incidence of CMV and HSV-1, possibly influenced by demographic covariates. Among hospitalized patients, males make greater SARS-CoV-2 antibody responses than females.

Humoral responses in coronavirus disease 2019 (COVID-19) are often of limited durability, as seen with other human coronavirus epidemics. To address the underlying etiology, we examined post mortem thoracic lymph nodes and spleens in acute SARS-CoV-2 infection and observed the absence of germinal centers and a striking reduction in Bcl-6 + germinal center B cells but preservation of AID + B cells. Absence of germinal centers correlated with an early specific block in Bcl-6 + T FH cell differentiation together with an increase in T-bet + T H1 cells and aberrant extra-follicular TNF-α accumulation. Parallel peripheral blood studies revealed loss of transitional and follicular B cells in severe disease and accumulation of SARS-CoV-2-specific “disease-related” B cell populations. These data identify defective Bcl-6 + T FH cell generation and dysregulated humoral immune induction early in COVID-19 disease, providing a mechanistic explanation for the limited durability of antibody responses in coronavirus infections, and suggest that achieving herd immunity through natural infection may be difficult.

The relationship between SARS-CoV-2 viral load and risk of disease progression remains largely undefined in coronavirus disease 2019 (COVID-19). Here, we quantify SARS-CoV-2 viral load from participants with a diverse range of COVID-19 disease severity, including those requiring hospitalization, outpatients with mild disease, and individuals with resolved infection. We detected SARS-CoV-2 plasma RNA in 27% of hospitalized participants, and 13% of outpatients diagnosed with COVID-19. Amongst the participants hospitalized with COVID-19, we report that a higher prevalence of detectable SARS-CoV-2 plasma viral load is associated with worse respiratory disease severity, lower absolute lymphocyte counts, and increased markers of inflammation, including C-reactive protein and IL-6. SARS-CoV-2 viral loads, especially plasma viremia, are associated with increased risk of mortality. Our data show that SARS-CoV-2 viral loads may aid in the risk stratification of patients with COVID-19, and therefore its role in disease pathogenesis should be further explored.

No abstract

No abstract

No abstract

No abstract

Offshore and inland river ship detection has been studied on both synthetic aperture radar (SAR) and optical remote sensing imagery. However, the classic ship detection methods based on SAR images can cause a high false alarm ratio and be influenced by the sea surface model, especially on inland rivers and in offshore areas. The classic detection methods based on optical images do not perform well on small and gathering ships. This paper adopts the idea of deep networks and presents a fast regional-based convolutional neural network (R-CNN) method to detect ships from high-resolution remote sensing imagery. First, we choose GaoFen-2 optical remote sensing images with a resolution of 1 m and preprocess the images with a support vector machine (SVM) to divide the large detection area into small regions of interest (ROI) that may contain ships. Then, we apply ship detection algorithms based on a region-based convolutional neural network (R-CNN) on ROI images. To improve the detection result of small and gathering ships, we adopt an effective target detection framework, Faster-RCNN, and improve the structure of its original convolutional neural network (CNN), VGG16, by using multiresolution convolutional features and performing ROI pooling on a larger feature map in a region proposal network (RPN). Finally, we compare the most effective classic ship detection method, the deformable part model (DPM), another two widely used target detection frameworks, the single shot multibox detector (SSD) and YOLOv2, the original VGG16-based Faster-RCNN, and our improved Faster-RCNN. Experimental results show that our improved Faster-RCNN method achieves a higher recall and accuracy for small ships and gathering ships. Therefore, it provides a very effective method for offshore and inland river ship detection based on high-resolution remote sensing imagery.