We describe the first set of ten microsatellite markers isolated in Pipistrellus abramus. The number of alleles per locus ranged from 7 to 13. The observed and expected heterozygosities values ranged from 0.486 to 0.971 and from 0.752 to 0.876, respectively. Three loci revealed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was found between loci pairs. These informative microsatellite markers will be a powerful molecular tool for studying the population genetic structure of P. abramus, as well as other species of this genus.
We constructed a genomic DNA library enriched for CA repeat motifs in Eonycteris spelaea. Nine microsatellite loci were isolated and tested on a population of 39 samples from Yunnan Province, China. These nine loci had three to 22 alleles per locus. Observed and expected heterozygosity values ranged from 0.079 to 0.963 and from 0.078 to 0.959. Two loci revealed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was found between loci pairs. These microsatellites can be a powerful molecular tool for population-level studies of E. spelaea.
The characteristics of male anurans' calls play an important role in mating success during the breeding season. In this study, we analyzed intra-and inter-individual variability of the advertisement calls of the pale-shouldered horned toad (Xenophrys boettgeri) in the field during its breeding season in Lishui, Zhejiang Province, China. The results showed that some acoustic parameters were significantly correlated with morphological features and air temperature. Most acoustic parameters including note numbers, call duration, and dominant frequency were static, whereas the call interval was intermediate. The CV b /CV w ratio was greater than 1.0 for all acoustic parameters analyzed, with between-individual variation being higher than within-individual variation. Furthermore, the acoustic parameter results of principle component analysis and discriminant function analysis indicated that note numbers and call interval are potentially useful for individual discrimination of X. boettgeri.
Fourteen polymorphic microsatellite loci were isolated in the pan-oriental narrow-mouthed toad, Microhyla ornata, which is one of the smallest anuran species. We genotyped one population from Lishui, Zhejiang Province, China (N = 29). The results showed that the mean number of observed alleles per locus was 11 (with a range from 8 to 15). Observed and expected heterozygosity values ranged from 0.250 to 0.931 and from 0.538 to 0.866 respectively. One locus (MO10) showed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was found among any pairwise loci. These highly informative microsatellite markers should be useful for population genetic analyses of M. ornata and other species in Microhylidae.
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