Abstract:An experiment was conducted to analyze thei-type lysozyme cDNA cloned from oyster (Crassostrea hongkongensis) and study the effect of temperature on the gene expression in an indoor, controlled constant-temperature environment. The cDNA was 634 bp with a 420-bp open reading frame, encoding 139 amino acids. A new conserved amino acid sequence, HNGGPRGC, was found in the protein sequence of the i-type lysozyme from C. hongkongensis. Real-time quantitative PCR (RTQ-PCR) was used to assess the expression of the i-type lysozyme gene in the digestive gland, smooth muscle, gills, mantle and labial palps of C. hongkongensis. The expression was the highest in the digestive gland and the lowest in the muscle. A thermal stress experiment was performed to study the effect of temperature on the expression of the i-type lysozyme gene. The expression was stable when water temperature ranged from 20 0 C to 25 0 C. The level of expression tended to decrease at 6 0 C or 13 0 C. A conclusion was drawed that the variations in temperature affect i-type lysozyme gene and immune function of C. hongkongensis.
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