Pythium species are soil-borne pathogens which can cause serious economic loss worldwide and threatening agricultural production. Traditional management methods like chemical fungicides are effective against Pythium spp. But as people pay more attention to human health and environmental issues, alternative methods that are ecofriendly and harmless to health are urgently needed. Currently, various approaches had been made including using natural extract, regulating planting conditions, using plant growthpromoting rhizobacteria and screening disease tolerance plants. Hereby, we review the recent achievements, particularly chemical and physical methods, biocontrol and host plant defense which can be used to control Pythium disease.
Crown gall disease caused by Agrobacterium tumefaciens causes economic loss in ornamental production in Japan. To breed a resistant rootstock, we used crown gall resistant Rosa 'PEKcougel' to hybridized with tetraploid of R. multiflora 'Matsushima No. 3'. In this study, we investigated the crown gall resistance characteristics in their progenies. In the needle prick test, eight tested progenies showed significantly smaller wounds than R. 'PEKcougel'. In the stem segment culture and opine assay, six progenies formed no callus tissue while three progenies formed calluses and had no opine detected. Further oncogene expression analysis was performed to evaluate crown gall disease resistance, since the low callus formation rate limited the application of opine assay. In the oncogene expression analysis, some progenies of tetraploid of R. multiflora 'Matsushima No. 3' showed a lower expression level of both ipt and iaaM than R. 'PEKcougel'. Furthermore, ipt expression had a significant positive relationship with tumor size. Taken together, these results suggest that ipt expression analysis can be used for evaluating crown gall disease resistance in rose progenies. Furthermore, eight progenies with strong crown gall disease resistance were confirmed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.