Poropuntius huangchuchieni is a medium-sized freshwater fish species, belonging to the Poropuntius genus, which has a broad geographic distribution spanning from southwestern China to Myanmar, Thailand, the Malay Peninsula, and Sumatra (Kottelat, 2013) with the Mekong River System being the most likely center of diversity for the genus.In China, the genus is naturally distributed in the Yunnan Plateau, the southeastern neighbor of the Qinghai-Tibetan Plateau, which consistently responded to the Qinghai-Tibetan Plateau's (QTP) uplifting during the Pliocene epoch, resulting in a dramatic change in the region's climate (Cheng et al., 2001;Ming & Shi, 2006). Consequently, the aquatic species in these regions may have experienced a driving force for diversification and speciation in evolutionary history, and
Spongicoloides zhoui sp. nov. (Crustacea: Decapoda: Spongicolidae), a species of deep-sea hexactinellid sponge-associated shrimp, is described based on specimens collected from the Zhenbei Seamount in the South China Sea. The new species is morphologically most similar to the Western Pacific congeneric species Spongicoloides iheyaensis Saito, Tsuchida & Yamamoto, 2006 in that the ischium of the third pereiopod is unarmed and the fixed finger of the third pereiopod is armed with small teeth on the distoventral margin. However, S. zhoui sp. nov. can be distinguished from S. iheyaensis in that its female antennal basicerite has three large spines on the distolateral margin. Molecular analyses based on nuclear histone H3, and mitochondrial cytochrome c oxidase subunit I (COI), 12S ribosomal RNA (rRNA), and 16S rRNA gene fragments confirmed the placement of S. zhoui sp. nov. within a clade of Spongicoloides/Spongiocaris species, and their sequence divergences were large enough to justify the recognition of this new species.
Alfalfa (Medicago sativa) is an important food and feed crop which rich in mineral sources. The WUSCHEL-related homeobox (WOX) gene family plays important roles in plant development and identification of putative gene families, their structure, and potential functions is a primary step for not only understanding the genetic mechanisms behind various biological process but also for genetic improvement. A variety of computational tools, including MAFFT, HMMER, hidden Markov models, Pfam, SMART, MEGA, ProtTest, BLASTn, and BRAD, among others, were used. We identified 34 MsWOX genes based on a systematic analysis of the alfalfa plant genome spread in eight chromosomes. This is an expansion of the gene family which we attribute to observed chromosomal duplications. Sequence alignment analysis revealed 61 conserved proteins containing a homeodomain. Phylogenetic study sung reveal five evolutionary clades with 15 motif distributions. Gene structure analysis reveals various exon, intron, and untranslated structures which are consistent in genes from similar clades. Functional analysis prediction of promoter regions reveals various transcription binding sites containing key growth, development, and stress-responsive transcription factor families such as MYB, ERF, AP2, and NAC which are spread across the genes. Most of the genes are predicted to be in the nucleus. Also, there are duplication events in some genes which explain the expansion of the family. The present research provides a clue on the potential roles of MsWOX family genes that will be useful for further understanding their functional roles in alfalfa plants.
The diploid Poropuntius huangchuchieni in the cyprinid family, which is widely distributed in the Mekong and Red River basins, is one of the most closely related diploid progenitor-like species of allotetraploid common carp, which was generated by merging of two diploid genomes during evolution. Therefore, the P. huangchuchieni genome is essential for polyploidy evolution studies in Cyprinidae. Here, we report a high-quality chromosome-level genome assembly of P. huangchuchieni by integrating Oxford Nanopore and Hi-C technology. The assembled genome size was 1021.38 Mb, 895.66 Mb of which was anchored onto 25 chromosomes with a N50 of 32.93 Mb. The genome contained 486.28 Mb repetitive elements and 24,099 protein-coding genes. Approximately 95.9% of the complete BUSCOs were detected, suggesting a high completeness of the genome. Evolutionary analysis revealed that P. huangchuchieni diverged from Cyprinus carpio at approximately 12 Mya. Genome comparison between P. huangchuchieni and the B subgenome of C. carpio provided insights into chromosomal rearrangements during the allotetraploid speciation. With the complete gene set, 17,474 orthologous genes were identified between P. huangchuchieni and C. carpio, providing a broad view of the gene component in the allotetraploid genome, which is critical for future genetic analyses. The high-quality genomic dataset created for P. huangchuchieni provides a diploid progenitor-like reference for the evolution and adaptation of allotetraploid carps.
The complete mitochondrial genome (16,497 bp) of the humpback grouper Cromileptes altivelis is first presented in this study. The gene arrangement and translate orientation of C. altivelis is identical to most vertebrates, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a putative control region. The overall nucleotide composition of the H-strand is 29.80% A, 26.23% T, 15.67% G and 29.02% C. The origin of the light-strand replication was identified between the tRNA-Asn and tRNA-Cys genes, while the termination associated sequence (TAS) and conserved sequence blocks (CSB1-3) were identified in the control region.
Global nuclear security is threatened by nuclear accidents and the purposeful use of nuclear weapons. Accordingly, atmospheric pollution prediction and control for nuclear accidents, including identifying sources in nuclear or radiological incidents, predicting hazards to persons and environments, and optimally controlling accident hazards, are current areas of nuclear security research. Source inversion, hazard prediction, and optimal control are three interrelated key issues for nuclear accident emergencies. Although progress has been made in hazard prediction for nuclear accidents since the 1970s and some source inversion methods were presented after the Fukushima nuclear accident, optimal control methods are rarely reported, source inversion methods are less practical, and prediction accuracy remains unsatisfactory. Thus, novel theories are required for optimal control and source inversion for nuclear accidents, and to develop methods for simulating the influences of radioactive plume dispersion and deposition under complex meteorological and terrain conditions. This work reviews the current progress, uncertainties, and research needs in nuclear security. In addition, a rapid source inversion method based on the Lagrangian model is developed and implemented in a test case. To address future challenges, an innovative architecture for Atmospheric Pollution Prediction and Optimal Control System for nuclear accidents (APPOCS) is proposed, and the perspectives are generalized to promote future research on nuclear accident hazard prediction and optimal control. At this time, forward-looking ideas and revolutionary perspectives are required to foster nuclear security research in the academic community.
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