Background
Grape seed proanthocyanidins (GSPs) have been shown to inhibit the progression of many cancers, including cutaneous squamous cell carcinoma (CSCC). Circular RNA (circRNA) is a key regulator for cancer progression. However, it is unclear whether GSPs can mediate the progression of CSCC by regulating circRNA.
Methods
Quantitative real-time PCR was conducted to determine the expression of hsa_circ_0070934, microRNA (miR)-136-5p and prenylated Rab acceptor family 2 (PRAF2). MTT assay and colony formation assay were used to assess cell proliferation. Cell cycle process and apoptosis were detected by flow cytometry, and cell migration and invasion were measured by transwell assay. Western blot analysis was utilized to examine protein expression. In addition, dual-luciferase reporter assay and RIP assay were used to evaluate the interaction between miR-136-5p and hsa_circ_0070934 or PRAF2. Subcutaneous xenograft models were constructed to explore the function of GSPs on CSCC tumor growth in vivo.
Results
GSPs could reduce hsa_circ_0070934 expression and inhibit CSCC cell proliferation, cell cycle process, migration, invasion, while promote apoptosis. Overexpressed hsa_circ_0070934 could reverse the suppressive effect of GSPs on CSCC cell progression. MiR-136-5p could be sponged by hsa_circ_0070934, and its overexpression also abolished the positively regulation of hsa_circ_0070934 on the progression of GSPs-induced CSCC cells. PRAF2 was a target of miR-136-5p, and its expression could be decreased by GSPs and increased by hsa_circ_0070934. The inhibitory effect of miR-136-5p on CSCC cell progression could be reversed by PRAF2 overexpression. Additionally, GSPs also could inhibit CSCC tumor growth in vivo.
Conclusion
Our data showed that GSPs regulated the hsa_circ_0070934/miR-136-5p/PRAF2 axis to restrain CSCC progression.
Evaluation systems for objects after laser removal have not been systematically established, yet they have great significance for repairing engineering coating materials. In general, this paper mainly focused on the surface quality after laser removal, which was simulated by thermal models and characterized by microscopic, residual chemical compositions, and wettability. The laser removal threshold was considered to be a key indicator to regulate removal precision. Here, the thermal model was used to explore the threshold was 15 J/cm2, and the ablation P was 308 W at 100 kHz. Besides, the surface structure was observed through simulation before experiments. It also predicted the effect of laser power and overlapping on surface roughness, and therefore provided the theoretical reference for experiments. Then, two groups of samples were set up of the surface of acrylic polyurethane coatings on 2024 aluminum alloy with and without anodizing treatment, respectively. After the coatings were removed, microscopic morphologies demonstrated a wavy undulation structure with little residue. Meanwhile, the increments of roughness were less than 0.5 μm and the surfaces were with nice wettability. Evaluating the surface quality from the above aspects is meaningful and helpful for the non-destructive cleaning of aircraft skin coatings.
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