We monitored submarine groundwater discharge (SGD) into the Werribee Estuary, Australia, using both chemical and physical methods. SGD occurred at hotspots where 222 Rn persisted through a 12 month survey period. A significant correlation between 222 Rn and NO
The microbial community composition and biogeochemical dynamics of coastal permeable (sand) sediments differs from cohesive (mud) sediments. Tide- and wave-driven hydrodynamic disturbance causes spatiotemporal variations in oxygen levels, which select for microbial generalists and disrupt redox cascades. In this work, we profiled microbial communities and biogeochemical dynamics in sediment profiles from three sites varying in their exposure to hydrodynamic disturbance. Strong variations in sediment geochemistry, biogeochemical activities, and microbial abundance, composition, and capabilities were observed between the sites. Most of these variations, except for microbial abundance and diversity, significantly correlated with the relative disturbance level of each sample. In line with previous findings, metabolically flexible habitat generalists (e.g., Flavobacteriaceae, Woeseaiceae, Rhodobacteraceae) dominated in all samples. However, we present evidence that aerobic specialists such as ammonia-oxidizing archaea (Nitrosopumilaceae) were more abundant and active in more disturbed samples, whereas bacteria capable of sulfate reduction (e.g., uncultured Desulfobacterales), dissimilatory nitrate reduction to ammonium (DNRA; e.g., Ignavibacteriaceae), and sulfide-dependent chemolithoautotrophy (e.g., Sulfurovaceae) were enriched and active in less disturbed samples. These findings are supported by insights from nine deeply sequenced metagenomes and 169 derived metagenome-assembled genomes. Altogether, these findings suggest that hydrodynamic disturbance is a critical factor controlling microbial community assembly and biogeochemical processes in coastal sediments. Moreover, they strengthen our understanding of the relationships between microbial composition and biogeochemical processes in these unique environments.
Cable bacteria represent a newly discovered group of filamentous microorganisms, which are capable of spatially separating the oxidative and reductive half-reactions of their sulfide-oxidizing metabolisms over centimeter distances. We investigated three ways that cable bacteria might interact with the nitrogen (N) cycle: (1) by reducing nitrate through denitrification or dissimilatory nitrate reduction to ammonium (DNRA) within their cathodic cells; (2) by nitrifying ammonium within their anodic cells; and (3) by indirectly affecting denitrification and/or DNRA by changing the Fe 2+ concentration in the surrounding sediment. We performed 15 N labeling laboratory experiments to measure these three processes using cable bacteria containing sediments from the Yarra River, Australia, and from Vilhelmsborg Sø, Denmark. Our results revealed that in the targeted systems, cable bacteria themselves did not perform significant rates of denitrification, DNRA, or nitrification. However, cable bacteria exhibited an important indirect effect, whereby they increased the Fe 2+ pool through iron sulfide dissolution. This elevated availability of Fe 2+ significantly increased DNRA and in some cases decreased denitrification. Thus, cable bacteria presence may affect the relative importance of DNRA in sediments and thus the extent by which bioavailable nitrogen is lost from the system.
During the past three years radiocarbon assay has emerged as a primary tool in the quantitative assignment of sources of urban and rural particulate pollution. Its use in several major field studies has come about because of its excellent (fossil/biogenic) discriminating power, because of advances in 14C measurements of small samples, and because of the increased significance of carbonaceous particles in the atmosphere. The problem is especially important in the cities, where increased concentrations of fine particles lead to pollution episodes characterized by poor visibility and changes in the radiation balance (absorption, scattering), and immediate and possibly long-term health effects. Efforts in source apportionment in such affected areas have been based on emissions inventories, dispersion modeling, and receptor modeling – ie, chemical and physical (and statistical) characterization of particles collected at designated receptor sites. It is in the last category that 14C has become quite effective in helping to resolve particle sources. Results are presented for studies carried out in Los Angeles, Denver, and Houston which incorporated 14C measurements, inorganic and organic chemical characterization, and receptor modeling. The 14C data indicated wide ranging contributions of biogenic and fossil carbon sources – eg, <10% to 60% contemporary (biogenic) in Houston – depending on meteorological, biological, and anthropological activity. The combined (chemical, isotopic, statistical) data point to sources such as vehicles, wood combustion, power plants, and vegetation.
The role of permeable sediments and subterranean estuaries as coastal nutrient filters is a question of key interest, particularly in areas with high nitrogen loadings. Here, we evaluated the effectiveness of a sandy subterranean estuary in cycling and removing nitrate using stable isotopes of N and O at natural (δ15N‐NO3− and δ18O‐NO3−) and enriched levels (15N). Isotopes were used in conjunction with flow through reactors under anoxic conditions to quantify (1) the overall enrichment factor (15ε) of nitrate removal processes which was then applied to estimate the in situ percentage of nitrate removal within the subterranean estuary and (2) the potential rates of denitrification, dissimilatory nitrate reduction to ammonium, and anammox. We found that 15ε varied between −24 and −34‰ and were positively correlated with nitrate concentrations and the percentage of organic carbon added to the sediments. Using these 15ε values in a Rayleigh distillation model resulted in an estimated average of 34% ± 14% nitrate removal within the subterranean estuary, less than half of the percentage estimated using the nitrate‐salinity mixing model (66% ± 28%). Denitrification was the most dominant nitrate removal pathway within the subterranean estuary with potential rates among the highest denitrification rates reported for both permeable and cohesive sediments. The contribution of dissimilatory nitrate reduction to ammonium showed significant seasonal variation while the rates of anammox were consistent throughout the study. We suggest that the spatial shift of the subterranean estuary is the most likely explanation for the seasonal differences in the rates of denitrification and dissimilatory nitrate reduction to ammonium.
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