This study aims to maintain the quality of pig semen for longer during storage at 15oC, in an effort to support artificial insemination programs with the addition of Bovine Serum albumin (BSA) to diluent Beltsville Thawing Solution (BTS). This study uses a completely randomized design with five treatment groups, each To = semen was diluted with BTS without the addition of BSA ; T1 = with the addition of 5 mg BSA/mL diluent; T2 = with the addition of 10 mg BSA/mL diluent; T3 = with the addition of 15 mg BSA/mL diluent; T4 = with the addition of 20 mg BSA/mL diluent. Each treatment was repeated five times so that the number of samples used was twenty-five. The diluted cement is stored at 15oC for 72 hours then observing the quality of cement includes: progressive motility, dead spermatozoa, abnormalities, and intact plasma membranes. The data obtained were analyzed by analysis of variance, if there were differences followed by Duncan’s test. The results showed, addition of BSA concentration of 10 mg/mL and 15 mg/mL of diluent gives the same effect on the quality of cement during storage and significantly better (p <0.05) when compared to the addition of 0 mg/mL, 5 mg/mL and 20 mg/mL diluents. It can be concluded, the addition of BSA 10 mg/mL BTS diluents can maintain the most optimal quality of pig semen against progressive motility, dead spermatozoa, abnormalities and intact plasma membranes.
Extracellular matrix (ECM) saat ini adalah salah satu yang paling umum digunakan sebagai perancah untuk prosedur rekayasa jaringan. Extracellular matrix (ECM) bisa berasal dari submukosa usus kecil atau vesica urinaria (kandung kemih) babi. Tujuan penelitian ini adalah untuk mengetahui tingkat kecocokan dan keamanan penggunaan vesica urinaria babi sebagai Extracellular matrix (ECM) terhadap proses kesembuhan luka terbuka pada tikus putih serta mengetahui perubahan yang terjadi pada daerah implan pasca pemberian bahan serbuk vesica urinaria yang diamati secara makroskopis. Tiga puluh dua ekor tikus putih jantan yang akan dipergunakan pada penelitian diadaptasi lingkungan selama 1 minggu dan diberikan pakan komersial (merk pellet 552) dan air minum ad libitum. Tikus dibagi menjadi dua kelompok masing-masing berjumlah 16 ekor. Kelompok I (perlakuan) yaitu 16 tikus pada daerah punggungnya dilukai dengan pisau scalpel sedalam 2 mm dan panjang 2 cm dan diberikan perlakuan bahan Extracellular matrix yang berasal dari vesica urinaria babi. Kelompok II (kontrol) yaitu 16 tikus diperlakukan sama dengan kelompok I dan tidak diberi perlakuan. Monitoring keamanan bahan matriks dilakukan pengamatan perkembangan kesembuhan luka pada 24 jam, hari ke 5, hari ke-10, dan hari ke-15 pasca operasi dengan pengamatan makros (kemerahan, bengkak, dan keropeng). Hasil pemeriksaan menunjukkan bahwa pemberian bahan Extracellular matrix mempercepat proses kesembuhan luka dibandingkan kelompok kontrol dengan tanda luka tertutup sempurna pada hari ke-15.
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