Aim and Objective: Currently, the use of ingredients from natural sources has gained great attention in cosmetic field especially for the development of new photoprotective formulations. Therefore, the present study aimed to evaluate the cosmetic potential of the methanolic crude extract and the ethyl acetate fraction of the medicinal halophyte Tamarix gallica (Tg) growing in the area of Tebessa in eastern of Algeria, by assessing their phenolic and flavonoid contents, photoprotective and antioxidant activities. Methods: The research approach consisted of determination phenolic and flavonoid contents of aerial parts via FolinCiocalteu and aluminum chloride methods respectively. The antioxidant activity was measured through two in vitro methods DPPH radical scavenging activity and total antioxidant capacity test (TAC). The in vitro photoprotective effect was performed according to the parameter SPF (Sun Protection Factor) by using UV spectroscopic method in UV-B region (290-320 nm). Results: The methanolic extract (Tg-MeOH) and ethyl acetate (Tg-EtOAc) fraction showed good antioxidant activity with IC50’s 14,05±0,66, 27,58±1,98 µg/mL respectively in DPPH test. Furthermore, both extracts displayed strong total antioxidant capacity (287.01±7.85, 246.7±1.12 mg AAE/g, respectively) in TAC test. Both extracts exhibited high photoprotective activity, with sun protection factor (SPF) values 37.03±0.22 and 36.08±0.03. The antioxidant and photoprotective activities of these extracts is probably related to polyphenols content (190.27±0.74 mg AGE /g and 121.77±1.29 mg AGE /g, respectively) , and flavonoids (78.75±0.06 mg QE /g and 58.67±1.19mg/g). Conclusion: Our finding show that extracts of Tamarix gallica L could be a promising source to be mixed as natural sunscreens and antioxidants agent into photoprotective cosmetic formulations.
Nicotiana glauca (Solanaceae), or tree tobacco, is found in dry arid climates of North America, Africa and Europe. It has been reported to have both toxic and medicinal properties. The main aim of this study was to analyze the phytochemical screening and quantitative estimation of polyphenols, flavonoids and flavonols of crude extracts from the leaves of Nicotiana glauca and to evaluate its in vitro antioxidant properties. Three different solvents were used to extract bioactive compounds from powdered leaves of Nicotiana glauca: dichloromethane (DCM), ethyl acetate (AE) and n-buthanol (n-BuOH). The three extracts were then subjected to qualitative phytochemical screening using standard procedures. Total phenolics, total flavonoids and total flavonols contents of the extracts were measured by Folin Ciocalteu and Aluminium chloride methods respectively. Furthermore, the antioxidant capacity evaluation was performed using the DPPH, ABTS, DMSO alcalin, Phenantroline, FRAP and CUPRAC methods. The three extracts were then subjected to qualitative phytochemical screening using standard procedures. These methods showed the presence of polyphenols and comarines in all extracts. Moreover, flavonoids, tannins, steroids and quinones were reported in the AE and n-BuOH extracts. In addition, alkaloids were seen to be present in DCM extract, while saponines and phlobatannins were absent in all extracts. The antioxidant capacity evaluation was performed using the DPPH, ABTS, DMSO alcalin, Phenantroline, FRAP and CUPRAC methods.Results using the DPPH method showed strong free radical scavinging activity for three extracts. This activity decreased with increasing concentration in the following order: n-BuOH>AE>DCM. In other assays, all extracts showed good antioxidant activity which decreased with increasing concentration in the following order: AE > n-BuOH > DCM. Extracts were compared with standards: BHT, BHA, Tanic acid and α-Tocopherol. The antioxidant of these extracts is probably related to polyphenols content (351.55±0.07, 284.98±0.08 and 133.8±0.06 mg/g), flavonoids (105.97±0.04, 164.44±0.07 and 1.18±0.005 mg/g) and flavonols (22.41±0.24, 18.75±0.46 mg/g) in AE and n-BuOH, respectively.As a conclusion, the results of the present study indicate that Nicotiana glauca leaves could be considered as a potential source of natural antioxidants.
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