First, we have found that the cold-tolerant peach cultivar was related to carbon metabolism, starch as well as sucrose metabolism, and phenylpropanoid anhydrase, and harpin proteins play important roles in cold tolerance of the cold-tolerant peach cultivar. Third, ten candidate protein-encoding genes were validated in RNA level by real-time PCR. What is the expected impact on horticulture?• Cold resistance is the main characteristic of life activity in fruit trees, which directly affects the introduction and resistance breeding. Peach is an extensively distributed fruit plant worldwide, which resistance among various cultivars. Therefore, it is useful to examine the underlying mechanisms of cold resistance of peach, so as to breed the cultivars with enhanced cold tolerance, and to offer related proteomic information.