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Reduced secretion and increased uptake of ApoB-containing lipoproteins may contribute to the low LDL-C observed in mice and humans with genetic ANGPTL3 deficiency.

In the past two decades, microfluidics has become of great value in precisely aligning cells or microparticles within fluids. Microfluidic techniques use either external forces or sheath flow to focus particulate samples, and face the challenges of complex instrumentation design and limited throughput. The burgeoning field of inertial microfluidics brings single-position focusing functionality at throughput orders of magnitude higher than previously available. However, most inertial microfluidic focusers rely on cross-sectional flow-induced drag force to achieve single-position focusing, which inevitably complicates the device design and operation. In this work, we present an inertial microfluidic focuser that uses inertial lift force as the only driving force to focus microparticles into a single position. We demonstrate single-position focusing of different sized microbeads and cells with 95~100% efficiency, without the need for secondary flow, sheath flow or external forces. We further integrate this device with a laser counting system to form a sheathless flow cytometer, and demonstrated counting of microbeads with 2200 beads/s throughput and 7% coefficient of variation. Cells can be completely recovered and remain viable after passing our integrated cytometry system. Our approach offers a number of benefits, including simplicity in fundamental principle and geometry, convenience in design, modification and integration, flexibility in focusing of different samples, high compatibility with real-world cellular samples as well as high-precision and high-throughput single-position focusing.

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"Huoshan shihu," derived from Dendrobium huoshanense, is a well-known, valuable, and rare Daodi herb. Because of its higher price and scarce resources, its related species D. officinale and D. moniliforme are usually presented as "Huoshan shihu" for sale. Fengdou, the processed form of D. huoshanense, its identification is particularly important. To effectively identify Fengdou of D. huoshanense and protect the Daodi herb, the morphological and microscopic characteristics of the stems of three Dendrobium species were examined. The results showed that macroscopic and microscopic features helpful for identification of the three species were the total lengths and internode numbers of stems, diameter and length of each internode, number of vascular bundles, and presence or absence of silica masses. The key features useful for distinguishing between D. huoshanense and its related species, and also between their Fengdou, were the stem lengths and the trends of change in diameter in different internodes. The results of the study indicated that a combination of macroscopic and microscopic identification techniques might conveniently and effectively be applied for identification of Dendrobium species. "Longtou Fengwei" is the main feature of the Daodi herb "Huoshan shihu," (D. huoshanense Fengdou) and reflects the wisdom behind the protection of Daodi herbs in ancient times.

Against the background of coordinated development of the Beijing–Tianjin–Hebei region, it is of great significance to quantitatively reveal the contribution rate of the influencing factors of urban land for optimizing the layout of urban land across regions and innovating the inter-regional urban land supply linkage. However, the interaction effects and spatial effects decomposition have not been well investigated in the existing research studies on this topic. In this study, based on the cross-sectional data in 2015 and using the spatial lag model, spatial error model and spatial Durbin model, we analyzed the relationship between urban land and regional economic development at the county level in the Beijing–Tianjin–Hebei region. The results show that: (1) there are endogenous interaction effects of urban land, and the growth of urban land in a county will drive the corresponding growth of urban land in neighboring counties; (2) the local population, average wages, highway mileage density, and actual utilization of foreign capital have positive effects on the scale of urban land in local and neighboring counties; local GDP in the secondary/tertiary sector and the urbanization rate have positive effects on local urban land scale, but negative effects on the urban land scale of neighboring counties; (3) the contribution degree of the direct effect is ranked as follows: GDP in the secondary/tertiary sector > total population > urbanization rate. The order of factors with a significant spatial spillover effect on the scale of urban land in neighboring counties is as follows: average wages > total population > highway mileage density. The GDP in secondary/tertiary sector, population, and urbanization rate are the main influencing factors for the scale of urban land at the county level in the Beijing–Tianjin–Hebei region. It is an important finding that average wages are the most prominent among the spatial spillovers. We should attach importance to the spillover effect of geographic space and construct an urban spatial pattern coordinated with economic development.

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