This paper is devoted to study the existence and uniqueness of solutions of a one parameter family of nonlinear fractional differential equation with mixed boundary value conditions. Riemann-Liouville fractional derivative is considered. An exhaustive study of the sign of the related Green's function is done.Under suitable assumptions on the asymptotic behavior of the nonlinear part of the equation at zero and at infinity, and by application of the fixed theory of compact operators defined in suitable cones, it is proved the existence of at least one solution of the considered problem. Moreover it is developed the method of lower and upper solutions and it is deduced the existence of solutions by a combination of both techniques. In some particular situations, the Banach contraction principle is used to ensure the uniqueness of solutions.
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