Objective To assess the viability of bovine ovarian tissue after cryopreservation through either slow freezing or vitrification, and to compare it to that of control tissue by performing morphological analyses. Methods The study included 20 bovine ovarian cortex fragments that were divided into control, vitrification, and slow freezing groups. Each group consisted of four fragments of the same ovary, two fixed without cultivation, and two fixed with cultivation. Tissues were evaluated based on follicular morphology immediately after heating and after 7 days of culture, and compared with the control group. Results A total of 240 fragments were analyzed, generating a sample of 1,344 follicles without cultivation and 552 with cultivation. When the non-cultivated samples were classified as non-atretic follicles, 572 were found in the control group, 289 in the vitrification group, and 373 in the slow freezing group, showing no significant differences. When classified as atretic, 46 follicles were found in the control group, 23 in the vitrification group, and 41 in the slow freezing group, also showing no statistical difference. In the post-culture sample, an evolution of the follicular stages could be observed. This finding was important to support that the follicles considered non-atretic in the non-cultivated group were actually viable in the morphological evaluation. Conclusion With no differences between the protocols, vitrification was shown to be an advanced and alternative method for patients who will undergo treatments that

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A ultrassonografia transvaginal (USTV) é um exame muito utilizado na propedêutica ginecológica. Um achado de espessamento endometrial à USTV, por exemplo, requer maior investigação, geralmente realizada pela histeroscopia diagnóstica, o método mais apropriado para avaliar a cavidade uterina. Estabelecer o perfil epidemiológico da mulher com achados de espessamento endometrial à USTV e investigar a correlação entre os achados desse exame e os provenientes da histeroscopia. Estudo transversal e prospectivo com delineamento observacional, baseado na análise histeroscópica de 79 pacientes cuja USTV constata espessamento endometrial. Das pacientes que apresentavam espessamento endometrial, foi obtida uma média de idade de 55,9 ± 11,4 anos. Dentre essas, 52 (65,8%) estavam na pós-menopausa. 31 mulheres (39,24%) eram assintomáticas e 48 (60,75%) relataram queixas de sangramento uterino anormal e dor pélvica 31 (39,24%). À histeroscopia, apenas 18 pacientes (22,8%) apresentavam de fato espessamento endometrial, sendo que 46 (58,2%) apresentavam pólipo endometrial, 9 (11,4%) apresentavam mioma submucoso e 10 (12,7%) não possuíam alterações na cavidade uterina. Apenas 3 (3,8%) das pacientes apresentaram lesões sugestivas de neoplasia maligna. As pacientes abordadas no estudo eram mulheres pós-menopausa, multíparas, com IMC acima da faixa considerada adequada e alta prevalência de comorbidades, em especial a hipertensão arterial sistêmica. Além de espessamento endometrial à USTV, queixavam de sangramento uterino anormal e/ou dor pélvica. Devido a esses fatores, essas mulheres possuíam um maior risco para neoplasias endometriais, o que justifica o acompanhamento e investigação por meio da histeroscopia.

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Introduction: Hysteroscopy is considered the gold standard in the evaluation of the uterus and its diseases. Its use as a diagnostic technique is an outpatient procedure, usually well tolerated by patients, allowing resumption of activities right after its completion. The teaching of the hysteroscopic technique tends to be time consuming, with a slow learning curve. Therefore, defining the impact of the histeroscopy's learning curve on the intensity of the pain felt by the patients can help improving the organization of hysteroscopy services, maximizing learning and reducing patient discomfort. Objectives: To analyze, with the construction of a learning curve, the possible relationship between the number of hysteroscopic procedures performed by the doctor and the intensity of pain reported by the patient during the exam. Methods: A prospective cohort study with 403 patients who underwent hysteroscopy in the Hospital Universitário São José (HUSJ). Results: 72.45% of the patients rated the pain as mild and 5.95% , as strong. Furthermore, Correlation between the learning curve and the pain reported by the patient during outpatient hysteroscopy procedure without anesthesia

objectives: To evaluate the impact of previous guidance on the hysteroscopy exam using multimedia resources (film) compared to verbal orientations only, on the pain intensity perceived by the patient during hysteroscopy without anesthesia. Methods: Randomized clinical trial, which included 187 women, who were referred for hysteroscopy. The patients in the experimental group (89) watched an explanatory video just before the exam. The control group (98), however, received only routine verbal guidance about the exam. The degree of pain perceived by all patients was measured 1 and 5 minutes after the procedure using Visual Analog Scale. results: In the experimental group, 1 minute after the examination, 29.2% of the patients reported mild pain, 24.7% moderate pain and 46.1% severe pain. In the control group, 28.6% of the patients reported mild pain, 29.6% moderate pain and 41.8% severe pain. In the analysis 5 minutes after the examination of the experimental group 60.7% of the patients reported mild pain, 28.1% moderate pain and 11.2% severe pain and the control group 62.3% of the patients reported mild pain, 26.5% moderate pain and 11.2% severe pain. Conclusions: The study was not able to demonstrate the relationship of pain perceived by patients with prior multimedia orientation, thus that, more studies are needed.