The macro and micromorphology of the leaf, stem, flower, root and rhizome of Artemisia vulgaris L. are presented with the aim of finding their diagnostic characters by which the plant can be easily identified in both the entire and powdered form. INTRODUCTION Artemisia vulgaris L. (Mugwort) is an aromatic perennial herb, distributed worldwide. It is mostly native to temperate North America, Europe, some in South America and North Africa to Siberia. Three Artemisia species (A. judaica L., A. scoparia Waldst. & Kit. And A. monosperma Del.) were listed and recorded in flora of Egypt. Moreover, two more species (A.vulgaris L. and A. verlotiorum Lamotte.) were newly added to Genus Artemisia (Boulos, 2002). Phytochemical studies of Artemisia vulgaris L. resulted in the isolation of sterols, triterpenes and flavonoids. Biological screening of total ethanolic extract of the aerial parts of the plant proved antibacterial (Singh et al., 2002; Sura et al., 2012), antifungal, analgesic, antiinflammatory and anticancer activities especially colon carcinoma (Rabe et al., 2002). In Iran the leaf anatomy of different Artemisia species was studied and the results showed that Leaf symmetry is very variable and the dorsiventral structure was only seen in A. vulgaris (Noorbakhsh et al., 2008). The literature review showed no reports concerning the macro-and micromorphology of the wild plant growing in Egypt. The present work covers macroand micromorphological study of the leaves, stem, flowers, rhizome and root with the aim of finding out the diagnostic features by which the plant can be easily identified in both entire and powdered forms. MATERIALS AND MATHODS Plant material Flowering and fruiting plant of Artemisia vulgaris L. was collected in the flowering and early fruiting stage on May 2013 from the plant growing wild in the irrigated canal bank, El-Qanater, Qalubiya, Egypt. The identity was kindly verified by Prof.
Background and Objective: Astragalus fruticosus Forssk. is an endangered wild Egyptian plant, with a poor germination ratio and rich in valuable phytochemicals. This study was aimed to develop an efficient micropropagation protocol as well as a screening of the cytotoxic, antidiabetic activities and phenolic profile of the micropropagated plantlets. Materials and Methods: Murashige and Skoog (MS) medium fortified with different concentrations of N 6 -Benzyl Amino Purine (BAP), Kinetin (Kn) and thidiazuron (TDZ) either singly or with auxins as 2, 4-Dichlorophenoxyacetic acid (2,4 D) was used for regeneration studies. Cell viability assay and "-glucosidase inhibition methods were used to evaluate cytotoxic and antidiabetic activities, respectively. Results: MS medium supplemented with 1 mg LG 1 BAP was optimum for direct shoot regeneration from explants. Regenerated shoots were rooted and complete plantlets were obtained and successfully acclimatized and grown under greenhouse conditions. Embryogenic callus was induced in MS medium with 1 mg LG 1 2,4 D showing 85.3% callusing capacity, small plantlets of 1.3-2.4 cm height were regenerated. Micropropagated plants showed strong cytotoxic and significant antidiabetic activities. Conclusion: Somatic embryogenesis and direct organogenesis are efficient regeneration systems that might be suitable for further biotechnological approaches as genetic transformation and somatic hybridization for improvement of seed germination ratios of this plant species.
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