One hundred thirty patients undergoing chemotherapy for different types of cancer attending Tikrit Teaching Hospital from January  2013 to Augusto 2013 were enrolled in this study. Their age were range between “11-83 years old”. 68 blood samples and 62 sputum samples were collected in this study. Candida spp. Infection was 14(20.5%) isolates from patients blood and  5(8.1%) isolates from patients sputum, were infections more in blood C.albicans 8(57.8%) , C.glabrata 3(21.4%) C.krusei 2(14.3%) and C.parasillosis while isolates from sputum were  3(60%) of C.albicans and C.glabrata 2(40%). The isolated were identified according morphological , Cultural and biochemical characteristic. Fungal isolates in present study produced various virulence factors hemolysin and protease production were produced in C.albicans 8(72.8), 11(100%) respectively, while C.glabrata  hemolysin production were  4(80%) and (40%) of protease production , while C.parasillosis isolate were produced protease and hemolysin. C.krusei  were produce only hemolysin 1(50%).