The relationship between human craniofacial morphology and the biomechanical efficiency of bite force generation in widely varying muscular and skeletal types is unknown. To address this problem, we selected 22 subjects with different facial morphologies and used magnetic resonance imaging, cephalometric radiography, and data from dental casts to reconstruct their craniofacial tissues in three dimensions. Conventional cephalometric analyses were carried out, and the cross-sectional sizes of the masseter and medial pterygoid muscles were measured from reconstituted sections. The potential abilities of the muscles to generate bite forces at the molar teeth and mandibular condyles were calculated according to static equilibrium theory using muscle, first molar, and condylar moment arms. On average, the masseter muscle was about 66% larger in cross section than the medial pterygoid and was inclined more anteriorly relative to the functional occlusal plane. There was a significant positive correlation (P less than 0.01) between the cross-sectional areas of the masseter and medial pterygoid muscles (r = 0.75) and between the bizygomatic arch width and masseter cross-sectional area (r = 0.56) and medial pterygoid cross-sectional area (r = 0.69). The masseter muscle was always a more efficient producer of vertically oriented bite force than the medial pterygoid. Putative bite force from the medial pterygoid muscle alone correlated positively with mandibular length and inversely with upper face height. When muscle and tooth moment arms were considered together, a system efficient at producing force on the first molar was statistically associated with a face having a large intergonial width, small intercondylar width, narrow dental arch, forward maxilla, and forward mandible. There was no significant correlation between muscle cross-sectional areas and their respective putative bite forces. This suggests that there is no simple relationship between the tension-generating capacity of the muscles and their mechanical efficiency as described by their spatial arrangement. The study shows that in a modern human population so many combinations of biomechanically relevant variables are possible that subjects cannot easily be placed into ideal or nonideal categories for producing molar force. Our findings also confirm the impression that similar bite-force efficiencies can be found in subjects with disparate facial features.
We describe a synthesis strategy for the preparation of lysine isotopologues that differ in mass by as little as 6 mDa. We demonstrate that incorporation of these molecules into the proteomes of actively growing cells does not affect cellular proliferation, and we discuss how to use the embedded mass signatures (neutron encoding (NeuCode)) for multiplexed proteome quantification by means of high-resolution mass spectrometry. NeuCode SILAC amalgamates the quantitative accuracy of SILAC with the multiplexing of isobaric tags and, in doing so, offers up new opportunities for biological investigation. Large-scale technologies for the comparative analysis of proteomes have become essential for modern biology and medicine (1, 2). To satisfy this increasing demand and boost statistical power, parallel processing of proteomes (i.e. multiplexing) is key. The ground was broken in this field about two decades ago by advances in both MS and stable isotope labeling (3-5). Since then, two distinct strategies have emerged, each with its own strengths and weaknesses. The first approach, stable isotope labeling by amino acids in cell culture (SILAC), 1 metabolically incorporates labeled amino acids into proteins and is considered the gold standard (6 -8). SILAC quantification provides unmatched accuracy, but simultaneous comparison of more than three proteomes, although possible, is not practical for most global studies (9, 10). A second, and increasingly popular, method is to chemically modify peptides originating from up to 10 different sources (a 3-to 5-fold boost in throughput over SILAC) with isobaric reagents (e.g. TMT or iTRAQ) (11)(12)(13)(14). The escalated throughput afforded by this strategy is, for many applications, essential; however, multiplexing via isobaric tagging comes at the cost of quantitative accuracy (15-17). Furthermore, because each sample is handled independently prior to labeling, systematic and random variation that occurs during sample processing cannot be accounted for as it is with metabolic labeling. Thus, experimenters designing a quantitative proteomics experiment must choose between accuracy and throughput.Recently we described a new approach that blends the SILAC and isobaric tagging methods (18). The strategy, neutron encoding (NeuCode), relies on the mass defects of atoms and their isotopes (19). In studies using two isotopologues of lysine, differing by 36 mDa, NeuCode SILAC quantified proteins as well as traditional SILAC, but it allowed deeper proteome coverage. NeuCode harnesses the exceptional resolving power of modern FT-MS systems so that quantitative information is only revealed by high-resolution scanning when desired, in either MS or tandem MS scans (20,21).Owing to the lack of suitable lysine isotopologues, our initial work with NeuCode SILAC offered only duplex quantification; consequently, we could only predict the utility of NeuCode SILAC (18). To test our supposition that NeuCode SILAC has the potential to combine the benefits of traditional SILAC and
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