Vesicular-arbuscular mycorrhizal fungi (VAMF) can reduce plant stress resulting from nutrient deficiencies, drought, and other factors. The objective of this work was to measure the effect of soybean \Glycine max (L.) Merr.] and grain sorghum [Sorghum bicolor (L.) Moench] rotation and fertilization on plant response and VAMF root colonization and diversity, and relate effects to soil environment. Fertilizer treatments consisted of no fertilizer, N, and manure. Rooting densities correlated with previous crop, VAMF colonization, and soil NO,. Root colonization by VAMF was affected by previous crop, rooting density, N fertilization, soil P, and water-filled pore space. Root colonization by VAMF ranged from 93% at 15 cm to 15% at the 120-cm soil depth. Root density and VAMF colonization were least when soybean was grown the previous year and manure was applied. Root colonization by VAMF for control, N, and manure treatments were 54, 53, and 30%, respectively, for continuous soybean and 61, 55, and 44%, respectively, for soybean from rotation plots. Root colonization by VAMF for control, N, and manure treatments were 69, 59, and 54%, respectively, for continuous grain sorghum and 56, 48, and 31%, respectively, for grain sorghum from rotation plots. These agricultural soils contained a diverse mixture of 26 VAMF species, which is probably a major factor in the region's soil productivity. Plants stressed due to cropping system or fertilizer practice have greater VAMF colonization and VAMF activity. A diverse VAMF population could increase the ability of VAMF to respond to different stresses. C OLONIZATION OF PLANT ROOTS by VAMF often reduces adverse effects of plant stresses (water, nutrient, and environmental factors) and has beneficial effects in maintaining crop yields. Grain sorghum and soybean are colonized by VAMF. Rotating crops and application of N fertilizer may affect VAMF colonization and final crop yield. Clegg (1982) and Roder et al. (1989a) showed that rotating these crops improved crop yield, compared with continuous cropping. They were unable, however, to explain the rotation effect based solely on N availability. Since VAMF colonize the root system and serve as a link between plant roots and soil, changes in cropping systems that affect the soil environment should affect both roots and VAMF. The rate and patterns of root growth vary with soil water, soil strength, bulk density, soil aeration, soil temperature, chemical and biological soil properties,