Humoral factors may be responsible for the hyperdynamic circulation seen in portal hypertension. Endotoxin, a peripheral arteriolar vasodilator, has been proposed to mediate this hemodynamic picture. We examined the pathogenic role of endotoxin in portal vein-ligated rats, a prehepatic portal hypertensive model with a well-developed hyperdynamic circulation. To this end, we (a) administered oral neomycin, a poorly absorbable antibiotic, at doses of 50 and 100 mg/day for 7 days and found no evident splanchnic hemodynamic effects of a 2-log-fold reduction of cecal aerobic bacterial flora as assessed by the radioactive microsphere technique in portal vein-ligated rats studied in the postanesthesia awake state; (b) assayed endotoxin in arterial samples using a quantitative limulus assay and found no evidence of endotoxinemia in PVL rats; (c) induced a state of endotoxin tolerance by repeated daily intraperitoneal injections of low-dose endotoxin and found no amelioration of the hyperdynamic state in portal vein-ligated rats. Our results do not support the hypothesis that endotoxin plays a major pathogenic role in the hyperdynamic circulation of this experimental model.
Since adult hemoglobin (Hb Ao) i s glycosylated post-translat i o n a l l y , we hypothesized t h a t Hb i n the f e t u s might r e f l e c t maternal glucose control. A chemical method (thiobarbituric acid) was used since Hb F co-elutes with glycohemoglobin (H~A I~) on cation chromatography. ~l o o d was obtained a t delivery from 13 normal and 9 insulin-dependent mother-infant p a i r s. Glycosyl a t i o n of Hb (G-Hb) was compared t o fructose standards and expressed a s nM fructose equiv. (FE)/mg Hb. meanfSD P1.
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