Elimination of low molecular weight proteins during sequential ultrafiltration/dialysis was studied in 29 uremic patients. Beta-2-microglobulin, retinol binding protein, free light chains lambda and kappa, Zn-alpha-2-glycoprotein, hemopexin, prealbumin, hemoglobin, albumin, acid alpha-1-glycoprotein, haptoglobin, alpha-1-antichymotrypsin, ribonuclease, lysozyme, amylase, non-specific esterase, and proteolytic activity were detected in all ultrafiltrates tested. The level of total protein and ribonuclease was determined in 36 crude ultrafiltrates from 23 patients. Concentrated ultrafiltrates were used to quantitate retinol binding protein, prealbumin, albumin, lysozyme, and amylase. Other proteins identified in the ultrafiltrates are present in trace amounts. The question was discussed whether ++inextensive but systematic loss of proteins during hemofiltration in chronic RDT might be the cause of patient homeostasis disturbances.
Isoenzymic patterns of 12 enzymes were investigated in extracts of eosinophils isolated from the peripheral blood of a patient with eosinophilic leukaemia using the poly‐acrylamide gel electrophoresis technique. The following enzymes were separated into at least two bands in either alkaline (pH 8.5) or acid (pH 4.5) pH: alpha‐naphthyl‐phosphate phosphatase, alpha‐glycero‐phosphate phosphatase, beta‐glycero‐phosphate‐phosphatase, phospho‐enolo‐pyruvate phosphatase, glucose‐1‐phosphate phosphatase, glucose‐6‐phosphate phosphatase, fructose‐6‐phosphate phosphatase, non specific esterase, lactic dehydrogenase, alpha‐glycero‐phosphate dehydrogenase, myeloperoxidase, acid ribonuclease. The acid phosphatases investigated exhibited differences in the substrate specifity. Phospho‐enolo‐pyruvate phosphatase show additional band of the phosphatase activity if compared with that demonstrated using other phosphatase substrates.
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