Eighty-three isolates of the rice blast fungus (Pyricularia oryzae) were tested with respect to genetic diversity and the possibility of race differentiation by electrophoresis. The fungus was genetically very heterogeneous. The isolates were differentiated into 6 races by pathogenicity on race differential varieties. There was little correlation between pathogenicity and zymogram types of one particular enzyme such as esterase, phosphatase or catalase. The isolates were divided into 14 groups by the combination of the zymogram types of the three enzymes. The isolates in the same group showed similar pathogenicity. A new method is proposed which differentiates the blast fungus races by the combination of zymogram type of enzymes. The details are discussed. ZusammenfassungDifferenzierung physiologischer Rassen des Erregers der Reisbraune, Pyricularia oryzae Cav. durch PAGE-Elektrophorese 83 Isolate des Erregers der Reisbraune, Pyricularia oryzae, wurden im Hinblick auf genetische Diversitat und die Moglichkeit zur Rassendifferenzierung mit Hilfe der Gelelektrophorese gepriift. Der Pilz war genetisch stark heterogen. Die Isolate wurden anhand der Pathogenitat auf einem Reistestsortiment in sechs Rassen differenziert. Zwischen der Pathogenitat und den Zymogrammtypen eines Enzyms wie Esterase, Phosphatase oder Catalase ergab sich kaum eine Beziehung. Die Isolate wurden durch Kombination des Zymogrammtypen der drei Enzyme in 14 Gruppen aufgeteilt. Die Isolate der gleichen Gruppe zeigten ahnliche Pathogenitat. Eine neue Methode wird vorgeschlagen und diskutiert, nach der die Rassen von Pyricularia oryzae anhand der Kombination von Zymogrammtypen differenziert werden konnen. U.S.
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