Background: An increasing global incidence of Mycobacterium Tuberculosis (MTB), and emergence of drug resistant strains of MTB, have critically promoted the urgent need to explore better effective methods that can rapidly detect MTB, and identify drug-resistant cases as recommended by World Health Organization report of 2008. Globally, multi-drug-resistant strains of MTB are increasingly becoming a huge public health issue, which has adversely affected the health care delivery of patients, especially in developing communities with limited functioning health care facility. Nonetheless, the aim and focus of the study is to evaluate the trend of rifampicin resistance strains of MTB among patients accessing care at the Federal Medical Centre Yenegoa, Bayalsa State.