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Spores of three strains of Clostridium botulinurn Type E were inoculated into 202 x 202 cans of beef stew and irradiated to permit the survival of a small number of spores per container. The cans containing survivors were incubated at 43 and 49°F to determine whether germination and outgrowth of surviving spores could take place. With all three strains at least some of the survivors were capable of developing to the point of producing swell spoilage and toxin at both temperatures.The significance. of these results in relation to extension of the refrigerated storage' life of food by radiation "pasteurization" is discussed.

The radiation resistance of spores of 6 strains of Type E CZostridium botulinum was determined in a beef stew substrate. The results are evaluated in terms of the minimum dose showing no spoilage for a 2 billion total inoculum level (20 cans each with 100 million spores per can) and in terms of a calculated radiation D value. The mean radiation D value for 6 strains is 0.132 megarad (range 0.125-0.138).A comparison with results for 6 strains of Type A and 5 strains of Type B shows that Type E strains possess about 45-5570 of the resistance to ionizing radiations of Types A and B. It is concluded that any radiation dose for food sterilization based upon the maximum resistance of Type A or B strains would provide ample protection against Type E strains. The uuswelled cans at the lowest radiation level showing no spoilage were free of toxin and recoverable Type E spores.