The following are summaries of seven of the papers presented at a Meeting of the Analytical Division held on July 16th-I7th, 1990, in ICI C and P Ltd., Runcorn, Cheshire.
Methods are described for the first reported successful isolation of the soluble form of the membrane-bound myo-inositol dehydrogenase(s) from Acetomonas oxydans. Conditions for optimum yields of active enzyme in a crude membrane-free protein extract were established. The relevant conditions are (a) cell rupture by solid-shearing, (b) solubilization of the complete 60000g pellet with sodium deoxycholate at pH7.2, (c) rapid separation of the released protein from sodium deoxycholate by gel chromatography.
An improved system for the phase-titration determination of kerosine adulteration in petrol has been developed. Calibration graphs are linear and are therefore especially efficacious for field applications. The range of kerosine adulteration covered (0-20% v/v) represents the extremes of adulteration found in the field.The method shows no significant dependence on temperature.
Paper-chromatographic and isotope-dilution analysis showed that considerable decomposition of ~-[l*C]mannose, ~-[~~C]ribose, and D-['~C]fructose occurred when these sugars were stored in vacuo in the freeze-dried state. The relative contributions to the decomposition of primary and secondary radiation effects are discussed. The results indicate that nonbonded water, retained by the sample after freeze-drying, may be responsible for most of the degradation, this being due to formation of hydrogen atoms and hydroxyl radicals by radiation. Storage of ~-[~*C]mannose in frozen dilute aqueous solution reduces the decomposition significantly and indicates a possible method of storing 14C-labelled carbohydrates of high specific activity for long periods.
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