A comparison of various cultural methods for use with microcolony and direct fluorescent-antibody (FA) techniques to detect salmonella was conducted using 102 naturally contaminated samples. The methods were: (a) a 27-h microcolony technique, (b) a 51-h selenite-F FA microcolony technique, (c) a 51-h tetrathionate brilliant green FA microcolony technique, and (d) a 53-h FA technique from a broth enrichment. All were compared to an F.D.A. approved modification of the AOAC method. The 51-h FA microcolony technique, which employed tetrathionate brilliant green broth and brilliant green agar plates, produced 4.9% false-positives and no false-negatives, and produced the greatest frequency of salmonellae detection.
An evaluation of the newly developed Clinical Sciences, Inc. Salmonellae Fluoro-Kit, which attempts to standardize the various aspects of the fluorescent-antibody (FA) procedure, was performed with 120 naturally contaminated human food, animal feed, and raw material samples. The Association of Official Analytical Chemists (AOAC) method for the detection of salmonellae was used as the control method. The Fluoro-Kit was found to be simple and conveniento to use. The results of this preliminary study show an industrially acceptable rate of recovery of salmonellae by using the Fluoro-Kit in comparison with the A.O.A.C. method. The Fluoro-Kit shows promise as a rapid, salmonellae FA screening method. Problems originally encountered in the application of the Fluoro-Kit are discussed. According to the manufacturer, strict adherence to the now revised procedures included in the Fluoro-Kit will control these problems.
Comparative studies of a modified fluorescent-antibody procedure and the 5 to 7 day method used by the Association of Official Analytical Chemists for the detection of
Salmonella
were made on 151 samples of wheat products and 183 swab samples. The agreement between the two methods for the 334 samples tested was 92.5%. Food samples yielded 94.7% agreement, whereas the swab samples yielded 90.7% agreement. There were 7.5% false positives for the total number of samples tested. No false negatives were obtained by using the fluorescent-antibody method.
An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide. Two hundred fifty naturally contaminated human food and animal feed samples, representing 647 trials, were tested by the FA method. A total of 18 different food and feed samples was used. The method used by the Association of Official Analytical Chemists (AOAC) for the detection of salmonellae was the control method. The percent agreement when comparing the FA slide method to the AOAC method ranged from 87.1 to 95.3%, depending upon the conjugated antisera used in comparative studies.
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