X-ray free-electron lasers (XFELs) enable obtaining novel insights in structural biology. The recently available MHz repetition rate XFELs allow full data sets to be collected in shorter time and can also decrease sample consumption. However, the microsecond spacing of MHz XFEL pulses raises new challenges, including possible sample damage induced by shock waves that are launched by preceding pulses in the sample-carrying jet. We explored this matter with an X-ray-pump/X-ray-probe experiment employing haemoglobin microcrystals transported via a liquid jet into the XFEL beam. Diffraction data were collected using a shock-wave-free single-pulse scheme as well as the dual-pulse pump-probe scheme. The latter, relative to the former, reveals significant degradation of crystal hit rate, diffraction resolution and data quality. Crystal structures extracted from the two data sets also differ. Since our pump-probe attributes were chosen to emulate EuXFEL operation at its 4.5 MHz maximum pulse rate, this prompts concern about such data collection.
A new operating mode has been developed for the Linac Coherent Light Source (LCLS) in which we shape the longitudinal phase space of the electron beam. This mode of operation is realized using a horizontal collimator located in the middle of the first bunch compressor to truncate the head and tail of the beam. With this method, the electron beam longitudinal phase space and current profile are reshaped, and improvement in lasing performance can be realized. We present experimental studies at the LCLS of the beam shaping effects on the free-electron laser performance.
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