Curcumin has been attributed with antioxidant, anti-inflammatory, antibacterial activities, and has shown highly protective effects against enteropathogenic bacteria and mycotoxins. Ochratoxin A (OTA) is one of the major intestinal pathogenic mycotoxins. The possible effect of curcumin on the alleviation of enterotoxicity induced by OTA is unknown. The effects of dietary curcumin supplementation on OTA-induced oxidative stress, intestinal barrier and mitochondrial dysfunctions were examined in young ducks. A total of 540 mixed-sex 1-day-old White Pekin ducklings with initial BW (43.4±0.1 g) were randomly assigned into controls (fed only the basal diet), a group fed an OTA-contaminated diet (2 mg/kg feed), and a group fed the same OTA-contaminated feed plus 400 mg/kg of curcumin. Each treatment consisted of six replicates, each containing 30 ducklings and treatment lasted for 21 days. There was a significant decrease in average daily gain (ADG) and increased feed : gain caused by OTA (P<0.05); curcumin co-treatment prevented the decrease in BW and ADG compared with the OTA group (P<0.05). Histopathological and ultrastructural examination showed clear signs of enterotoxicity caused by OTA, but these changes were largely prevented by curcumin supplementation. Curcumin decreased the concentrations of interleukin-1β, tumor necrosis factor-α and malondialdehyde, and increased the activity of glutathione peroxidase induced by OTA in the jejunal mucosa of ducks (P<0.05). Additionally, curcumin increased jejunal mucosa occludin and tight junction protein 1 mRNA and protein levels, and decreased those of ρ-associated protein kinase 1 (P<0.05). Notably, curcumin inhibited the increased expression of apoptosis-related genes, and downregulated mitochondrial transcription factors A, B1 and B2 caused by OTA without any effects on RNA polymerase mitochondrial (P<0.05). These results indicated that curcumin could protect ducks from OTA-induced impairment of intestinal barrier function and mitochondrial integrity.
The influence of calcium source (limestone and oyster shell) and particle size (<0.1 mm; 0.85 to 2 mm) on laying performance, egg quality, and bone properties were examined in laying ducks by a 2 × 2 factorial arrangement of treatments. Longyan females (288) with similar BW at 24 wk of age were randomly allotted into 4 treatments, each with 6 replicates of 12 individually caged birds and studied over the following 12 wk. Particle size affected egg weight and feed conversion (P < 0.05). Large particle size increased shell breaking strength, albumen height, Haugh unit, and shell content of phosphorus and magnesium (P < 0.05), but had no effect on egg shape, yolk color, shell thickness, or the weight proportion of shell. There were no effects of particle size on tibial properties: dry defatted weight, calcium content, or breaking strength. Limestone increased albumen height, shell content of calcium and phosphorus, and the breaking strength of tibia (P < 0.05). It is concluded that limestone with a large particle size provided for superior productive performance, egg quality, and bone characteristics and is more suitable than oyster shell for practical applications.
In June 2009, disease symptoms on peanut (Arachis hypogaea) were observed in several fields in Huoping county, Guangdong Province. The characteristic symptoms were black rot of the basal stems and roots, with many orange-brown fruiting bodies on the diseased parts. Entire vines eventually wilted and died. The disease incidence reached as much as 30% in some fields, causing severe yield losses. A fungus was consistently isolated from the edge of lesions and grown on potato dextrose agar at 25°C. Mycelia were white and floccose. Conidia were cylindrical to oblong-ellipsoidal, hyaline, one-celled, and measured 3-15 · 1-5 lm. Perithecia were glabrous apart from a number of rhizoidal hyphae, ostiolate and with a neck. The asci were cylindrical, thin-walled, stalk 5-27 lm long, 101-161 lm tall and 10-15 lm in diameter, without discernible apical structures, not evanescent, eight-spored. Ascospores were uniseriately arranged, pale, globose to ellipsoidal, and 7-16 · 7-12 lm.
The purpose of this study was to estimate the selenium (Se) requirement of egg-laying ducks based on daily egg production and the selenoprotein glutathione peroxidase (Gpx). Five-hundred and forty laying ducks were divided into six treatments, each containing six replicates of 15 ducks. The birds were caged individually and received a Se-deficient basal diet (0.04 mg/kg) or diets supplemented with 0.08, 0.16, 0.24, 0.32, 0.40 mg/kg Se (as sodium selenite) for 6 months. The experiment consisted of two periods: an early-laying period of 2 months and the peak-laying period of 4 months. Egg production and feed intake were recorded daily. At the end of the experiment, blood samples were drawn for determination of Gpx activity in plasma (Gpx3) and in erythrocytes (Gpx1). Hepatic Gpx1 activity and relative expression of Gpx1 mRNA were also determined. Eggs (n = 6) were sampled for quality determination and Se content at the end of the experiment. The activities of plasma Gpx3, erythrocyte Gpx1 and liver Gpx1 increased in a quadratic manner (P < 0.001) with increasing supplemental Se. The mRNA abundance of hepatic Gpx1 increased linearly (P < 0.001) with dietary Se supplementation. Egg shell thickness was significantly reduced in the ducks fed 0.44 mg Se/kg (P < 0.05), indicating that higher dietary Se tends to compromise egg shell quality. Yolk and albumen contents of Se increased linearly (P < 0.0001) with dietary Se supplementation. Using quadratic broken line models, the Se requirement for daily egg production was 0.18 mg/kg for early-laying ducks and 0.24 mg/kg for peak-laying ducks; for optimal function of Gpx (peak-laying ducks), it was 0.37 mg Se/kg.
From June 2009 to November 2010, a disease was observed on peanut (Arachis hypogaea L.) in Ganzhou City, Jiangxi Province, China. Infected plants initially exhibited yellow leaves, then defoliated, and finally wilted and died. Basal stems, pegs, pods, and roots became black and rotted, with many orange-brown spherical fruiting bodies emerging on the lesions. Disease incidence reached as high as 30% in some plots, especially in those covered with plastic sheets after planting to control weeds. Isolations from 68 diseased plants were conducted on potato dextrose agar (PDA) amended with streptomycin sulfate and incubated at 25°C. A fungus was consistently isolated from the edge of the lesions. Mycelia grew at a linear rate of 3.9 mm per day on PDA at 25°C forming a pale buff, floccose colony with abundant orange-red perithecia. Perithecia were globose to pyriform, ostiolate and with a short neck, and measured 151 to 353 × 141 to 313 μm. Asci were narrowly cylindrical to clavate, thin walled with a short stalk, measured 100 to 160 μm high and 9 to 15 μm in diameter, and were eight spored. Ascospores were uniseriately arranged, globose to ellipsoid, nonseptate, thick walled, hyaline to pale yellow, and measured 7 to 16 × 7 to 12 μm. Scanning electron microscopy revealed that the wall of the ascospores had cerebriform ornamentation. Conidia were cylindrical to oblong-ellipsoidal, hyaline, most one celled, and measured 3 to 15 × 1 to 5 μm, aggregating in a gelatinous mass on the tip of the conidiogenous cell, which usually arose directly from the vegetative mycelium. The fungus was identified as Neocosmospora vasinfecta var. africana (anamorph Acremonium sp.) (1). The rDNA internal transcribed spacer (ITS) region of the fungus was amplified with universal primers ITS1/ITS4 and sequenced. The sequences of a representative isolate (No. N-JXLN02) were submitted to GenBank (Accession No. JF708085) and BLAST searches showed 99 to 100% similarity with sequences of N. vasinfecta deposited in GenBank (Accession Nos. HM461900, HM461901, and AY381142 ). Pathogenicity tests were conducted on 36 2-week-old peanut seedlings, cv. Yueyou No. 13, in plastic pots. Plants were inoculated by drenching the soil near the shoot with a mixed suspension of conidia and ascospores (105 spores per ml). Control plants were treated with sterile water. All plants were then incubated in a moist chamber at 25 ± 2°C. Fifteen days after inoculation, all inoculated plants showed lesions on basal stems and black root rot similar to that observed on naturally infected plants. No disease was observed on control plants. The pathogen was reisolated from infected tissues. To our knowledge, this is the first finding of Neocosmospora foot rot of peanut in Jiangxi Province, where outbreaks of this disease have been observed in several counties. The pathogen will pose a threat to peanut, which is a major oil crop in China. It has been previously reported in Taiwan (2) and recently in Guangdong Province, though the subspecies of the pathogen was not identified in the latter case (3). References: (1) P. F. Cannon and D. L. Hawksworth. Trans. Br. Mycol. Soc. 82:673, 1984. (2) J. W. Huang et al. Plant Pathol. Bull. 1:203, 1992. (3) R. Pan et al. Plant Pathol. 59:1172, 2010.
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