Dendritic cells (DC) are highly specialized antigennon-transduced DC. Whereas transduction of marker presenting cells (APC) that initiate and modulate immune genes (LacZ or enhanced green fluorescence protein responses. They are essential for naive T cell activation, (EGFP) ) did not alter their potent allostimulatory activity, but may also play roles both in central and peripheral toler-DC transduced with CTLA4Ig exhibited striking reductions ance. Blockade of costimulatory pathways that provide the in cell surface staining for CD86, but not MHC class II, and crucial second signal for lymphocyte activation is one stratwere poor stimulators of T cell proliferation and cytotoxic egy to augment the potential tolerogenicity of DC. Here, in T lymphocyte (CTL) responses. In addition, they induced vitro propagated DC were transduced using an adenoviral alloantigen-specific T cell hyporesponsiveness. They were (Ad) vector to express the gene encoding cytotoxic T detected, following local injection, in significantly increased lymphocyte antigen 4-immunoglobulin (CTLA4Ig), which numbers in the lymphoid tissue of unmodified allogeneic blocks interaction of CD80 and CD86 on DC with CD28 on recipients. This is the first report of the functional properties T cells. Supernatants of AdCTLA4Ig-transduced DC strikof DC genetically engineered to express CTLA4Ig. ingly inhibited mixed leukocyte reactions (MLR) induced by

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