No abstract

An electrochemical sensor was prepared using a platinum (Pt) electrode modified with a binary layer of poly(1,5-diaminonaphthalene) (p-1,5-DAN) and platinum nanoparticles (PtNPs). The obtained sensor was investigated using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), square wave voltammetry (SWV), and electroactive surface area measurements (EASA). The PtNPs/p-1,5-DAN/Pt electrode was applied for individual and simultaneous determination of Paracetamol (PCT) and moxifloxacin hydrochloride (MFH) in aqueous solutions of Britton–Robinson buffer (BRB). Under optimized conditions, the PtNPs/p-1,5-DAN/Pt electrode exhibited excellent sensing ability toward the oxidation of PCT and MFH with linear responses with ranges of 1–1000 μM and 10–1000 μM and detection limits of 0.09 μM and 0.10 μM, respectively. PtNPs/p-1,5-DAN/Pt exhibits good repeatability, reproducibility, and acceptable stability and was found to be applicablefor simultaneous determination of PCT and MFH in pharmaceutical formulations and spiked biological fluids.

A new, simple, accurate, and specific RP-HPLC stability method for determining bilastine was developed and validated. The proposed method was administered using C18 BDS Hypersil thermo column (4.6 × 250mm i.d), 5 µm particle size with a combination of potassium dihydrogen phosphate buffer pH 6.0: acetonitrile: methanol (50:25:25) as the mobile phase at a wavelength of 220nm. The retention time was 3.9 min for bilastine. The calibration plot was linear over the concentration range of 14.4–33.6µg/ml bilastine with LOD and LOQ of 0.04 and 0.11µg/ml, respectively. The technique was validated for linearity, sensitivity, accuracy, precision, and robustness. Percent recoveries were observed to be nearly 100%. The validated method was used for determining bilastine in Pharmabilast(R) tablets. The technique could be appropriate for routine evaluation at laboratories.

A new, simple, accurate and specific RP-HPLC stability indicating method for determination of bilastine has been developed and validated. The proposed method has been administrated by C18 BDS Hypersil thermo column (4.6 x 250 mm i.d), 5 μm particle size with a combination of potassium dihydrogen phosphate buffer pH 6.0: acetonitrile: methanol (50:25:25) as mobile phase at wave length 220 nm. The retention time has been 3.9 min for bilastine. Calibration plot has been linear over the concentration range 14.4-33.6 μg/ml bilastine with LOD and LOQ of 0.04 and 0.11 μg/ml, severally. The technique has been validated for linearity, sensitivity, accuracy, precision and robustness. Percent recoveries have been observed near to a hundred percent with slight change. The validated method has been applied for determination of bilastine in Pharma-bilast(R) tablets. The technique could be appropriate for routine evaluation at laboratories.