Background Vaginal candidiasis is frequent in women of reproductive age. Accurate identification Candida provides helpful information for successful therapy and epidemiology study; however, there are very limited data from the Vietnam have been reported. This study was performed to determine the prevalence, species distribution of yeast causing vaginal discharge and antifungal susceptibility patterns of Candida albicans among symptomatic non-pregnant women of reproductive age. Methods Vaginal discharge samples were collected from 462 women of reproductive age in Hanoi, Vietnam between Sep 2019 and Oct 2020. Vaginal swabs from these patients were examined by direct microscopic examination (10% KOH). CHROMagar™ Candida medium and Sabouraud dextrose agar supplemented with chloramphenicol (0.5 g/l) were used to isolate yeast, and species identification was performed using morphological tests and molecular tools (PCR and sequencing). Antifungal susceptibility testing was determined according to the Clinical and Laboratory Standards Institute guidelines (M27-A3 and M27-S4). Results The prevalence of vaginal yeast colonization in non-pregnant women was 51.3% of 462 participants. Nine different yeast species were identified. Among these isolates, C. albicans (51.37%) was the most frequent, followed by C. parapsilosis (25.88%), C. glabrata (11.37%), C. tropicalis (4.31%), C. krusei (3.92%), C. africana (1.57%), Saccharomyces cerevisiae (0.78%), C. nivariensis (1 isolates, 0.39%), and C. lusitaniae (1 isolates, 0.39%), respectively. Among C. albicans, all 46 isolates were 100% susceptible to micafungin, caspofungin, and miconazole. The susceptibility rates to amphotericine B, 5-flucytosine, fluconazole, itraconazole and voriconazole were 95.65, 91.30, 91.30, 82.61 and 86.95%, respectively. Conclusions The prevalence of VVC among symptomatic non-pregnant women of reproductive age in Vietnam was higher than many parts of the world. The high frequency of non-albicans Candida species, which were often more resistant to antifungal agents, was a notable feature. Resistance rates of vaginal C. albicans isolates to antifungal agents was low. Our findings suggest that continued surveillance of changes in species distribution and susceptibility to antifungals should be routinely screened and treated.

Background and Objectives: Identification of pnemococcal serotypes and antimicrobial resistance provides helpful infor- mation for the use of suitablevaccines and antibiotics; however, very limited data is available on these issues in Vietnam. The present study aimed to find the serotype distribution and drugresistance patterns of Streptococcus pneumoniae isolated from unvaccinated children less than 5 years of age with pneumonia at a province in centre Vietnam. Materials and Methods: A total of 126 clinical pnemococcal strains isolated from unvaccinated children less than 5 years of age with pneumonia at theNghe An province, Vietnam between Nov 2019 and Mar 2021. All strains were identified using conventional microbiological method, VITEK® 2 Compactsystem, specific PCR and sequencing. The serotypes and antimicrobial resistance patterns of pnemococcal strains were determined using the multiplex PCRassays and VITEK® 2 Compact system. Results: The results showed that, eight different pneumococcal serotypes were identified. The  most  common  serotypes were 19F (67.46%), followed by23F (10.32%), 19A (9.52%), 6A/B (3.17%), 15A (2.38%), 9V (3.17%), 11A (1.59%) and 14 (0.80%), respectively. More than half of the pneumococcal strainswere non-susceptible to penicillin. The resistance rate to ceftriaxone and cefotaxime were 41.3% and 50.8%. The percentage of pneumococci strains resistantto clarithromycin, azi- thromycin, erythromycin, cotrimoxazole, tetracyclin, and clindamycin were more than 93% of all strains. All pneumococcal serotypes were highly resistant to clarithromycin, azithromycin, erythromycin, cotrimoxazole, and clindamycin. Conclusion: Our findings showed high antibioticresistance rates of the strains causing pneumococcal pneumonia, mostly macrolide resistance, among unvaccinated children.

Objectives: To evaluate the outcome of selective reconstructive anterior cruciate ligament (ACL) surgery using the All-inside technique with a four-strand semitendinosus or gracilis autograft tendon. Subject and methods: 34 patients were diagnosed with a partial tear of the ACL and underwent selective reconstructive surgery at the Orthopedic Center, Military Hospital 103 from November 2018 to September 2020. Lysholm knee scale and IKDC class were used to access post-operatively clinical outcomes. Stability was evaluated using the anterior drawer test, the Lachman test, the Pivot shift test, and the Rolimeter measure at 20o of knee flexion. Results: The pre-operative and post-operative Lysholm scores were 49.5 ± 18.4 and 94.47 ± 6.81, respectively (p < 0.001).         Pre-operatively, most patients were classified as C and on the IKDC score. At the final follow-up, IKDC scores included 22 class A, 10 class B, 2 class C (p < 0.001). The pre- and post-operative laxity measured by Rolimeter were 5.15 ± 1.5 mm and 1.74 ± 1.34 mm, respectively (p < 0.001). Pre-operatively and post-operatively, the Pivot-shift was absent in all cases. Conclusion: Selective reconstruction of the injured ACL bundle restores knee stability and function. All-inside technique can be used to reconstruct the rupture ACL bundle with many advantages. Longer follow-up is needed to fully evaluate clinical outcomes after selective reconstruction in partial ACL tears. * Keywords: All-inside; Partial tear; Selective reconstruction.