investigations of angiogenesis biological characteristics in the post-infarction zone may be of great importance not only for understanding the causes of stroke development, but also for more qualitative treatment planning. And although prognostic and therapeutic principles are only being formed, achievements in understanding the processes of angiogenesis have already been used in clinical practice, and their assessment, perhaps, will be applied in routine clinical activities.The aim of the investigation was to evaluate the possibilities of using immunobiological preparation Cryocell-Cryocord in the treatment of ischemic stroke on the basis of the findings obtained in the course of studying morphological characteristics of brain tissue and the condition of the rats cerebral capillaries in experiment.Materials and Methods. 120 white outbred rats were used in our investigation. They were divided into 4 groups, with 30 animals in each: intact rats without focal cerebral ischemia (FCi) and treatment; rats after FCi and with no treatment; rats after FCi with standard treatment; and rats after FCi with standard treatment complemented by Cryocell-Cryocord. A comparative morphometric analysis of the sensorimotor cortex structural components and the newly formed blood vessels in the necrotic area has been carried out during the period of cerebral infarction progression. The ultrastructure has been examined by an electron microscope.Results. inclusion of the immunobiological preparation Cryocell-Cryocord into the standard treatment scheme for ischemic stroke has been found to trigger angiogenesis in the infarcted area and to preserve the blood-brain barrier structure, which favored the recovery of the lost functions.Conclusion. The immunobiological preparation Cryocell-Cryocord has been shown to possess pronounced angioprotective properties and to stimulate endogenous angiogenesis, which allows it to be included into the treatment scheme for ischemic stroke.
This paper deals with the concept of microliter sensing of human blood samples by waveguide dielectrometry at microwave frequency (39.5 GHz). The methods, research protocol, and basic results derived from the permittivity measurements are presented. Here we summarize the results of our 5-year research on developing a multidisciplinary approach to the characterization of erythrocytes dielectric response, in parallel with biochemical studies of their receptor-membrane activity, within the scope of a case–control study for in vitro diagnostics of acute and chronic neurological conditions.
In the present work we propose a new integrated approach to characterization of cellular reactivity using human erythrocytes in health and disease. We have applied two methods -microwave waveguide-based dielectrometry (39.5 GHz) and osmotic fragility technique based on photometry -to monitor receptor-specific response of blood cells caused by the beta-blocker exposure. Our results showed that dielectric response of erythrocytes depends on the physiological state of blood donors that is in good agreement with the degree of their beta-receptors activity (beta-ARM index). We found that druginduced changes in complex permittivity and the values of beta-ARM index are inversely related. The proposed approach is intended to supplement current cellular analysis used for healthcare, which will allow obtaining more information about health status of the patients at the cellular level.
The aim of the study was the β-adrenergic activity of peripheral blood erythrocyte membranes in the acute period of ischemic stroke (IS). Peripheral blood erythrocytes are the most informative biological tissue for studying many pathogenetic mechanisms, and the work of their membrane-receptor complex (MRC) can very well reflect the functional state of the whole organism, one of the most important criteria for assessing is the adrenergic activity of cytoplasmic membranes in the pathogenesis of IS. This study solves the problem of the finding of the features of changes in the adrenoceptors system in the acute period of IS in the dynamics of the treatment of humans cryopreserved cord blood serum (CCBS). The main scientific results: the obtained results indicate a reduced ability of adrenoceptors (ARs) on erythrocyte membranes to bind blockers due to the desensitisation effect, which is observed from the 1st day of the disease. In the onset of IS, there is a significant increase in the values of β-adrenergic activity of membranes (β-ARM) of erythrocytes in 2.4 times compared with the control. Maximum levels of the indicator (42.43±3.64 CU) are observed in patients with initially severe disease, which indicates significant stress of the sympathoadrenal system (SAS) in these patients. On the 10th day of the disease, there was a decrease in β-ARM relative to the values obtained during hospitalisation. Still, comparing the value of the indicator on the 10th day of IS in both groups, there was an apparent, more significant decrease in β-ARM in the 2nd group of patients, which additionally received CCBS. The use of CCBS contributes to a more pronounced degree of recovery in neurological functions (6.9%) and faster stabilisation of the structure of MRCs in erythrocytes in the form of reducing the values of β-ARM to 16.61±2.86 CU (p<0.05). The area of practical use of the research results: the results of the study can be used to diagnose and treat of the IS. An innovative technological product: A comprehensive approach to assess the β-ARM of peripheral blood erythrocytes in patients with IS by determining the degree of changes in osmotic resistance of erythrocytes (ORE) under the action of adrenergic drugs (AD) is informative and can be used to diagnose of IS. The use of neurotrophic factors is a potentially new method of treating this pathology. Scope of application of the innovative technological product: in the clinical practice of neurological departments for the treatment of patients with strokes
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