The described glucose biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide, which functions as a mediator. The electrode is further modified by the enzyme glucose dehydrogenase, which is immobilized on the electrode's surface through electropolymerization with m-phenylenediamine. The enzyme biosensor was optimized and tested in model glucose samples. The biosensor showed a linear range of 500–5000 mg L−1 of glucose with a detection limit of 210 mg L−1 (established as 3σ) and response time of 39 s. When compared with similar glucose biosensors based on glucose oxidase, the main advantage is that neither ascorbic and uric acids nor paracetamol interfere measurements with this biosensor at selected potentials.
An alcohol dehydrogenase-based biosensor was prepared and tested for its use to determine ethanol in beer. The biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide and immobilized with a biocatalytic layer containing the enzyme. Function of the enzyme biosensor was tested in model ethanol samples, in which it showed a linear range of 15-120 g•L −1 with a detection limit of 3.3 g•L −1 (established as 3σ) and response time of 19 s. In a potential window from -0.2 to +0.45 V, interferences of both ascorbic and uric acids were negligible. Several types of marketed beers of Czech provenance were selected and subjected to measurements under optimized conditions but without any pretreatment of real samples. When compared with the reference method (gas chromatography), the results were in quite good agreement for beers of the pale lager type but higher contents of ethanol were indicated in the samples of dark lager beers.
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