In present study, we identified the chemical composition of essential oil which was isolated from the leaves of Vitex rotundifolia using gas chromatography-mass spectrometry (GC-MS) analysis for the first time. A total of eighteen compounds were identified from essential oil, including sclareol (29.01 %); sandaracopimarinal (16.51 %); abietadiene (15.65 %); androsta-4,6dien-3-one, 17-hydroxy-, (17β)-(8.12 %); abietal (6.45 %); dehydroabietan (5.02 %); verticiol (4.89 %) as major constituents. Furthermore, the antibacterial activity of the essential oils isolated from of the leaves of studied species has also been evaluated in the first time. The results proved that the essential oils could inhibit the growth of six pathogenic bacterial strains which the diameter of the growth inhibition zone of S.
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