We investigated the phenolic composition, radical scavenging activity, and antimicrobial activity of grapevine leaf extracts from two red grape varieties, Vranac and Merlot (Vitis vinifera L.). The extracts were prepared from healthy grapevine leaves and those infected by Plasmopara viticola (downy mildew). The phenolic composition of the grapevine leaf extracts was determined using spectrophotometric assays and reverse-phase high performance liquid chromatography (RP-HPLC). The radical scavenging activity of grapevine leaf extracts was determined by the 2,2-diphenyl-1-picrylhydrazyl assay, and their antimicrobial activity was determined by microwell dilution tests. The total phenolic content was higher in healthy grapevine leaf extracts than in infected grapevine leaf extracts. The RP-HPLC analysis detected significant amounts of flavonols, phenolic acids, and flavan-3-ols, and small amounts of stilbenes in the grapevine leaf extracts. Compared with the infected grapevine leaf extracts, the healthy grapevine leaf extracts were richer in flavonols, phenolic acids, and flavan-3-ols, but had lower stilbenes contents. All extracts showed strong free radical scavenging activity, which was strongly correlated with the total phenolic content (R(2) = 0.978). The extracts showed a stronger antimicrobial activity towards Gram-positive bacterial strains than towards Gram-negative bacterial strains and yeast. The phenolic compounds in grapevine leaves were responsible for their strong radical scavenging and antimicrobial activities. Together, these results demonstrate that grapevine leaves have high nutritional value and can be used as a fresh food and to prepare extracts that can be used as additives in food and medicines.

Wine production generated significant quantities of waste -grape pomace (seeds, skin and stems of grapes). In these vinification by-products a significant amount of bioactive compounds such as phenol compounds can still be found. The phenol composition and radical scavenging activity of grape pomace obtained during winemaking from the red wine grape of Vranac variety (Vitis vinifera L.) were investigated to determine their usable values. Reversephase high performance liquid chromatography (RP-HPLC) assays were used for determination of the phenolic composition of pomace extracts and the DPPH test was done for their radical scavenging activity. The oil obtained from the pomace was subjected to GC/MS analysis in order to establish its fatty acid composition. As correlation calculations showed (R 2 =0.9732), phenol compounds are responsible for strong radical scavenging activities of the tested extracts (1.160 ± 0.03 mg DM/ml). Grape pomace oil with a high degree of of unsaturation (over 88%) plays an important role for human health. The phenolic rich extract and grape pomace oil can be used as additives in food and pharmacy industries.

How consumers will behave when buying wine depends on many factors, among which economic, social, demographic, psychological and situational ones are highlighted. Research in wine consumer attitudes in Serbia is aimed at creating appropriate marketing strategies. This paper analyses the influences of demographic factors on wine consumption.Special attention was paid to the impact of the wine characteristics that affect the decision to buy the local or imported wines with geographical origin. It should be added that wine tourism is particularly important in increasing the sales of wine by direct and indirect means.To this aim, 1.705 wine buyers and consumers were interviewed during 2015. Based on Chi-test, it has been proven that the age group affects the decision on the purchase of wine. Understanding consumer motivation is possible through an adequate marketing approach which will provide increased sales of domestic wine and the quicker development of the national market.

Fifteen commercial wines produced from international and autochthonic varieties of Vitis vinifera L. cultivation of different Balkan winegrowing subregions were studied for their antimicrobial activity against six Gram-positive (Clostridium perfringens, Bacillus subtilis, Staphylococcus aureus, Listeria inocua, Sarcina lutea and Micrococcus flavus) and six Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Shigella sonnei, Klebsiella pneumonia and Proteus vulgaris) bacteria. The concentrations and types of phenolic compounds responsible for antibacterial activity in wines were investigated by HPLC and spectroscopic methods. The correlation between amounts of phenolics and antibacterial activities of investigated wines were studied by application of statistical (PCA, factor and cluster) analyses. This study gives the possibility to predict the biological quality of the wine from the same cluster towards bacteria without "wet" analysis. Obtained results can to be useful both to wine producers for the formation of market price of wine, and to wine consumers in choosing quality red wine with high content of polyphenols.

Consumer behaviour when purchasing wine is the result of a complementary operation of the large number of different factors, which may include economic, geographic, social, psychological, and other. Discovering consumer preferences for wine and their buying behavior would allow the application of an appropriate marketing strategy to increase the sales of wine. Special attention was given to one of the most promising new demographic segments that likes to buy-Millennial generation. The main purpose of this research is to find out if the wine attributes of the Millennials are different from other generations in southern Serbia. Results of this research are shown that when choosing wine, the quality of wine has the biggest impact on consumers, rather than others wine attributes. The paper also asserts that, the wine industry should particularly focus on marketing to Millennial age group, as this segment has a high willingness to experiment.