Heterotrimeric G-proteins are molecular switches that regulate numerous aspects of cellular physiology by transducing the signals from G protein-coupled receptors (GPCRs). In the basal state, the Gα-subunits of the heterotrimeric G proteins are GDP-liganded (the inactive form) and bind to the βγ-complex. GPCRs can activate guanine nucleotide exchange on the Gα-subunits to produce the active, GTP-bound state. GoLoco domains present in many proteins play important roles in multiple heterotrimeric G protein-dependent activities, physically binding the Gα-subunits of the Gαi/o class. In most cases GoLoco binds exclusively to the GDP-loaded form of the Gα-subunits. Our biochemical and genetic experiments as well as structural modeling show that the poly-GoLoco protein Pins binds to both the GDP-and GTP-forms of Drosophila Gαo. We identify the Pins GoLoco domain 1 as necessary and sufficient for the unusual interaction with Gαo-GTP. We further pinpoint the central Lysine residue present in this domain as responsible for the interaction. Molecular modeling suggests that the side chain of this Lysine points directly into the guanine nucleotide-binding pocket of Gαo, stabilizing the extra negative charges of the γ-phosphate group of GTP. Such a positively charged amino acid is unique in the Drosophila GoLoco proteome, but is conserved in several GoLoco domains of other organisms. We conclude that Pins, through its GoLoco domain 1, is a target for Gαo-mediated GPCR signaling.
Receptors of the Frizzled family initiate Wnt liganddependent signal transduction cascades controlling multiple steps in organism development and are highly conserved in animal evolution. Misactivation of the Wnt/ Frizzled signaling underlies many cases of cancerogenesis. Frizzled receptors possess seven transmembrane domains and their signaling depends on trimeric G proteins in various organisms. However, as Frizzled proteins constitute a distinct group within the superfamily of G protein-coupled receptors (GPCR), and as Frizzled signaling can apparently be G protein-independent in some experimental setups, the GPCR nature of Frizzled receptors has been questioned. Here we demonstrate that human Frizzled receptors can directly bind the trimeric Go protein in a pertussis toxin-sensitive manner. Furthermore, addition of Wnt ligands elicits Frizzled-dependent guanine nucleotide exchange on Go. An excess of secreted Frizzledrelated protein, a known antagonist of the Wnt/Frizzled pathways, inhibits Go activation, as does pretreatment of Go with pertussis toxin. These experiments provide a biochemical proof of the GPCR activities of Frizzled receptors. They also establish an in vitro assay of monitoring Frizzled activation by Wnt ligands, applicable for the high-throughput agonist/antagonist screening.
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