Phytochemical investigation of Azorella madreporica led to the isolation of four known compounds and an unknown chalcone. The structure of the new compound was identified by spectroscopy, including two-dimensional NMR techniques and comparison with published spectral data. The antioxidant activity of chalcone (compound 1) was measured using the 1,2-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay, and the bioactivity was evaluated against five bacteria (Mycobacterium smegmatis ATCC 14468, clinical isolates of Staphylococcus aureus, Klebsiella granulomatis, Morganella morganii and Escherichia coli) and four cancer cell lines. Docking studies with the tested cancer related proteins revealed nearby values of energy between doxorubicin and compound 1. Besides, protein-ligand interactions correlate with these energy values.
The present study was aimed at evaluating the antibacterial activity of mulinane and azorellane diterpenes isolated from the Andean plants Azorella compacta and A. trifoliolata and semisynthetic derivatives against reference and multidrug-resistant strains. The results revealed that the semisynthetic compound 7-acetoxy-mulin-9,12-diene (5) exhibited antibacterial activity against reference and multidrug-resistant strains of Staphylococcus aureus and moderate antimycobacterial activity against Mycobacterium smegmatis ATCC 14468.
The aim of this study was to synthesize a series of novel and known dihydrocarvone-hybrid derivatives (2–9) and to evaluate mycelial growth activity of hybrid molecules against two strains of Monilinia fructicola, as well as their toxicity. Dihydrocarvone-hybrid derivatives have been synthesized under sonication conditions and characterized by FTIR, NMR, and HRMS. Antifungal efficacy against both strains of M. fructicola was determined by half maximal effective concentration (EC50) and toxicity using the brine shrimp lethality test (BSLT). Among the synthesized compounds, 7 and 8 showed the best activity against both strains of M. fructicola with EC50 values of 148.1 and 145.9 µg/mL for strain 1 and 18.1 and 15.7 µg/mL for strain 2, respectively, compared to BC 1000® (commercial organic fungicide) but lower than Mystic® 520 SC. However, these compounds showed low toxicity values, 910 and 890 µg/mL, respectively, compared to Mystic® 520 SC, which was highly toxic. Based on the results, these hybrid compounds could be considered for the development of more active, less toxic, and environmentally friendly antifungal agents against phytopathogenic fungi.
Introduction: The Calgary Family Medicine (FM) Residency Program implemented a competency-based curriculum in 2012 (Triple C). To meet the College of Family Physicians' accreditation standards, the Program also implemented a new assessment program. Field notes (FNs) were introduced to record feedback and to provide data for decisions around Resident competence and progress. Validation of inferences from data collected in field notes is sparse, particularly in relation to how the data can be extrapolated to competence and professional practice. This study investigates the quality and trustworthiness of FNs when their content is used to make decisions about a Resident's competence. Methods: Assessment data from over 3100 FNs, 99 inprogram progress decisions, and scores on the the SOOs and SAMPs components of the College of CFPC Certification Examination in FM were analysed for 16 randomly selected Residents who had successfully completed the Urban FM Residency Program in Calgary under the Triple C Curriculum. Six independent raters (FM community preceptors) were recruited to review copies of the same sets of FNs that were originally used by in-program preceptors to assess Resident progress. 2 independent blinded raters were randomly assigned to each set of FNs. Raters were asked to use FN data to decide on Resident progress, and indicate their level of confidence in their decisions. This was compared with Peer Review under the responsibility of Universidad Nacional Autónoma de México. the actual in-program progress decision previously made for each Resident. Quality of FNs was assessed using a Formative Feedback Evaluation Tool (FFET), and were scored 1-5. Results: The quality of the FN data was found to be suboptimal (mean 2.27). The consistency of raters' progress decisions was high (89%). Correlation analyses indicated a significant weak positive relationship between quality of FNs and raters' confidence, r(196) = .201, p = .005; a significant moderate positive linear relationship between number of FNs and raters' confidence, r(196) = .30, p < .001; and a significant moderate positive linear relationship between total number of FNs received by a resident and the residents z-scores in the SAMPS component of the CFPC exam, r(14) = .55, p = .026 Conclusion: The results provide evidence supporting the validity of assessment decisions based on Field Note data. The quality and number as well as the quality of the FNs appears important in supporting the trustworthiness of summative progress decisions.
RESUMEN: Para que se desarrolle el iris, se requiere una especificación de la capa periférica de la copa óptica a un destino no neuronal y además la migración de células mesenquimales perioculares. Nuestro objetivo fue reconocer los cambios histológicos de los derivados periféricos de la copa óptica y mesénquima periocular, como también reconocer la presencia del morfógeno Sonic hedgehog (Shh) en las capas que constituyen el esbozo de iris. Se utilizaron 15 ratones hembras (Mus musculus) adultas jóvenes gestantes. Se realizó eutanasia con tiopental sódico. Los embriones y fetos de 12, 14,5 y 17 días post-coital (dpc) fueron procesados con técnica histológica e inmunohistoquímica con anticuerpo anti-Shh (scbt, H-160, conejo) con dilución 1:100 en PBS. A los 12 dpc, se observa una cópa óptica que presenta capas retinianas interna y externa, y el iris no se observa. Entre el cristalino y el ectodermo superficial se identifican 4 capas de células mesenquimales. A los 14,5 dpc, el iris contiene dos capas epiteliales (interna y externa) que se continúan con las capas neural y pigmentaria de la retina. Se observan 8 capas de células mesenquimales. A los 17 dpc, la capa epitelial interna del iris presenta un segmento más elongado con inmunotinción positiva a Shh y otra parte que constituye un epitelio de células cilíndricas simples negativas a este anticuerpo. La capa epitelial externa presenta el mismo epitelio inmunonegativo. Las capas de la retina también son positivas, como también la periferia del cristalino. No esta formado el iris ni tampoco el cuerpo ciliar. La inmunopositividad en el cristalino, en el primer segmento de la capa interna del esbozo del iris y en la capa ganglionar retinal a los 17 dpc, se relaciona con la diferenciación tardía del iris y con los ojos cerrados de las crías al nacimiento.
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