In freshwater fish with external fertilization, sperm sampling can be contaminated with urine, which triggers motility and gives rise to decreased fertilization success. The maintenance of freshwater fish in hyperosmotic conditions may reduce urine production and improve sperm quality. Thus, the aim of this work was to verify if acute exposure to various NaCl concentrations improves sperm quality in the yellowtail tetra Astyanax altiparanae. Spermiation was induced using a single dose of carp pituitary gland (5 mg kg-1) and the males were maintained at various NaCl concentrations: NaCl 0.00% (control), NaCl 0.45% (hypoosmotic), NaCl 0.9% (isosmotic) and NaCl 1.0% (hyperosmotic) for 6 h at 26 °C. Sperm was collected and verified for activation by urine and motility traits. At 0.00%, 0.45%, and 0.90%, the sperm was motile just after sampling, indicating activation by urine. Surprisingly, at hyperosmotic conditions, no activation was observed. Other sperm and motility parameters did not show any statistical differences, including sperm viability (P = 0.7083), concentration (P = 0.9030), total motility (P = 0.6149), VCL (curvilinear velocity; P = 0.1216), VAP (average path velocity; P = 0.1231) and VSL (straight-line velocity; P = 0.1340). Our results indicate that acute maintenance at hyperosmotic conditions eliminates sperm activation by urine and maintains sperm quality. Such a new procedure is interesting for both basic and applied sciences, including reproductive practice in fish.
Agradeço a minha orientadora, Lilian Rose Marques de Sá, pela oportunidade, paciência e conhecimento que me passara. Obrigada pela confiança e por acreditar no meu trabalho. À Karen, Liura, Alex e Letícia que me ajudaram e me aconselharam durante todo esse tempo. Aos pesquisadores Luara Lucena Cassiano, Carlos Massatoshi Ishikawa e Leonardo Tachibana por terem me recebido mais uma vez no Instituto de Pesca e terem me ensinado e ajudado em meu projeto. Aos veterinários da Clínica de Bovinos e Pequenos Ruminantes do Hospital Veterinário da FMVZ-USP por terem colaborado com o projeto. Ao pesquisador Ricardo Harakava pela paciência e generosidade de ter me recebido em seu laboratório e colaborado com a pesquisa. Ao técnico Mauro do Laboratório de Histologia do VPT por ter colaborado com a montagem das lâminas histológicas. Aos fornecedores dos peixes por terem colaborado com o projeto. Agradeço ao Programa de Pós-Graduação em Patologia Experimental e Comparada pelo financiamento e oportunidade. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Processo nº 88887.595546/2020-00. KIDA, V.R. Caracterização de processos patológicos que acometem peixes-de-briga-Siamês (Betta splendens) no estado de
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