No abstract

Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase chain reaction (qualitative PCR) analytical sensitivity was 101 DNA copies/μL and found 4.8% (14/290) positive for OvHV-2. The quantitative polymerase chain reaction (qPCR) analytical sensitivity was 100 DNA copy/μL and 5.9% (17/290) positivity, with 47.1% (8/17) of the positive samples presenting histological evidence of non-purulent meningo-encephalitis. The qualitative PCR products (422 bp of the ORF75 region) were sequenced and submitted to phylogenetic analysis. Identity matrices showed 100% similarity in OvHV-2 samples obtained in this study and those recovered from GenBank, corroborating other studies.

Bovine coronavirus (BCoV) is one of the main aetiological agents of gastroenteritis in calves, causing significant economic damage to livestock. This study aims to characterise BCoV genetically on the basis of the N gene. A total of 114 faecal samples from beef and dairy calves with or without clinical symptoms of diarrhoea from five Brazilian states (São Paulo, Minas Gerais, Santa Catarina, Mato Grosso and Bahia) were evaluated between 2008 and 2015 by technique of Semi‐nested RT‐PCR for gene N and genealogical analysis. Of the 114 samples analysed, 14.91% (17/114) were positive. BCoV was detected in 22.72% (10/44) of the animals with diarrhoea and in 10% (7/70) of asymptomatic animals. BCoV was identified in calves from rural properties located in all of the regions sampled. Genealogical analysis showed that the Brazilian sequences of BCoV for the gene which codes for the N protein can be broken down into two distinct clusters, and the samples from this study were closely linked to Asian strains. These results contribute to the molecular characterization of BCoV in Brazil and are the first report of the circulation of BCoV in the states of Santa Catarina and Bahia.

32 a RAIB Reunião Anual do Instituto Biológico Os resumos são apresentados na forma de pôsteres, durante o evento, e publicados no Suplemento da revista "O Biológico", online. A Comissão Organizadora empenhou-se para que houvesse uma programação que permeasse pela sanidade, nosso ícone de conhecimento, trazendo novas propostas dos nossos convidados. Os temas abordados são: Criomicroscopia Eletrônica, Reconstrução Tridimensional em MET, Programa Agente local de Inovação-SEBRAE, As qualidades humanas essenciais para culturas organizacionais saudáveis, Gestão de empreendimentos agroflorestais com uso de imagens aéreas, Producão orgânica de leite: princípios e desafios, Desafios da Cadeia da batata, Desafios para o controle da brucelose x acreditação de laboratórios, Controle de qualidade para laboratório, Avanços na produção de antígenos para suportar o Programa nacional de controle e erradicação da brucelose e tuberculose. Agradecemos à Dra. Ana Eugênia de Carvalho Campos, pelo total apoio no transcorrer de nosso trabalho, durante a organização da 32ª RAIB. À Comissão Organizadora, que não mediu esforços para o sucesso do evento. Ao Centro Administrativo, pela colaboração emprestada. Também, à Silvana D'Agostini, pela sua sempre colaboração. Não poderíamos deixar de agradecer aos nossos apoiadores e patrocinadores, FUNDEPAG, Laboratórios BRUCH LTDA e THERMOFISHER.