Considering the limited availability of technology for the production of rice vinegar and also due to the potential consumer product market, this study aimed to use alcoholic fermented rice (rice wine (Oryza sativa L.)) for vinegar production. An alcoholic solution with 6.28% (w/v) ethanol was oxidized by a submerged fermentation process to produce vinegar. The process of acetic acid fermentation occurred at 30 ± 0.3°C in a FRINGS  Acetator (Germany) for the production of vinegar and was followed through 10 cycles. The vinegar had a total acidity of 6.85% (w/v), 0.17% alcohol (w/v), 1.26% (w/v) minerals and 1.78% (w/v) dry extract. The composition of organic acids present in rice vinegar was: cis-aconitic acid (6 mg/L), maleic acid (3 mg/L), trans-aconitic acid (3 mg/L), shikimic + succinic acid (4 mg/L), lactic acid (300 mg/L), formic acid (180 mg/L), oxalic acid (3 mg/L), fumaric acid (3 mg/L) and itaconic acid (1 mg/L).Keywords: submerged fermentation; microfiltration; acetic acid bacteria; vinegar.Practical Application: Vinegar rice produced by a submerged fermentation process from alcoholic fermented rice.

Rice wine was made from broken rice (by‐product of rice processing), which was hydrolyzed by amylolytic enzymes, producing syrup with high content of dextrose equivalent (51.04% DE). The must presented 164 g/L of glucose (15 ºBx) and pH of 2.67 was fermented for 22 h at 25C by Saccharomyces cerevisiae. Fermented alcoholic (rice wine) reached yield value of 74.94% (Y1), 91.71% (Y2) and productivity (Pp) of 2.85 g/Lh in alcohol, with alcohol concentration of 62.8 g/L, 28.0 g/L of glucose, 2.5 °Bx and pH of 3.0. The kinetic parameters – Yp/s 27.75%, μN 0.008 h−1, gt 3.99 h, PN 0.13 logUFC/mLh – were determined. The wine contained a total of 11 volatile compounds identified by GC‐FID, 7 alcohols (methanol, ethanol, n‐propanol, n‐butanol, iso‐amylic alcohols, iso‐butanol and glycerol), 2 aldehydes (acetaldehyde and acetol), 1 ester (ethyl acetate) and 1 ketone (acetone). Practical Applications The product obtained showed potential features to be employed not only as alcoholic beverage, but also as a raw material in the production of other products such as vinegar, adding value to rice processing. Considering the production outlook and the amount of generated by‐product, it is a lower cost substrate for such application. The characterization of volatile flavor compounds might be made use not only for a better understanding of this new‐type product, but also for the further utilization in other wine or alcohol production.

This study aimed to verify the need for minerals and vitamins to increase the production of cell mass by acetic acid bacteria (AAB) isolated from the vinegar industry (086/06) and standard strain (Acetobacter aceti CCT 2565). Five minerals (Mo, B, Zn, Fe, and Mn) and eight vitamins (p-aminobenzoic acid, thiamine, niacin, pantothenic acid, pyridoxine, biotin, cyanocobalamin, and inositol) were tested in a fractional factorial design. To prepare the inoculum, different compositions of MYP (mannitol, yeast, and peptone) medium were tested. The most adequate medium was mannitol 25 g/L, yeast extract 0.625 g/L, and peptone 0.375 g/L. Through contour curves, it was determined that strain 086/06 needed supplementation with minerals Mo, B and Mn and vitamins p-aminobenzoic acid, pyridoxine and cyanocobalamin. Standard strain CCT 2565 needed supplementation of all minerals and vitamins studied, except inositol. The lower requirement of micronutrients for high cell multiplication of the 086/06 strain may be related to the adaptation of strain 086/06 to industrial production conditions.

In a hydrothermal process for the gelatinization of the rice starch, it was studied the rate of hydration, temperature and time. An incomplete 3 3 factorial design was used to evaluate the effects of temperature, enzyme/substrate relation and liquefaction time, with dextrose equivalent (DE) as the response variable. The maximum value obtained was 14.69% DE at 89ºC, with 0.21% (w/w) of enzyme to substrate in 22 minutes. For saccharification, it was studied enzyme/substrate ratios (0.6, 2.4, 4.2 and 6.0% (w/w)) at 60ºC at a pH of 4.30, yielding 49.23, 69.65, 65.12 and 74.67% DE, respectively, after 72 hours. The syrup had a content of 12.78, 16.87, 15.96 and 17.87% of glucose (w/v), pH 2.60-2.71. The starch content converted into glucose after liquefaction was 61.80% and after saccharification ranged from 75.16 to 77.05% for the relation enzymes substrate tested.

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