Mycorrhizal Colonization Affects the Survival of Vetiveria zizanioides (L.) Nash Grown in Water Containing As(III)The presence of arsenic (As) in water is of great public concern. Arsenic exists in three common valence states viz., As(0) metalloid arsenic, As(III) (arsenite) and As(V) (arsenate). Arsenite [As(III)] is the most toxic form among arsenicals which, predominates in anaerobic conditions, generally in flooded soils and in the water with high BOD. Experiments were conducted to investigate the effect of As(III) on the mycorrhization in vetiver (Vetiveria zizanioides (L.) Nash) grass in hydroponics. Studies showed significant alteration in the mycorrhizal colonization in the roots of vetiver exposed to higher concentrations of As(III) starting from 1.0, 2.0, 3.0, 4.0 to 5.0 mg/L prepared in 5% Hoagland nutrient solution without addition of phosphate ions. Considerable reduction in the mycorrhizal intensity (M) was observed in all the treatment sets as compared to the control suggesting a negative impact of the As(III) on the mycorrhizal association. Simultaneously, the study also showed that, As(III) is toxic to the vetiver plants having mycorrhizal association however plants with non-mycorrhizal (cleansed) roots were found to be able to survive for a longer period exposed to As(III).
Nigrosine Black (Acid Black-2) is popularly used in tanning industries and a huge amount of this dye comes out with effluent of tanning industries, this dye reached near water bodies, and what impact produced on their aquatic life not reveal yet, Hence a study was plan to find out the impact of Nigrosine black dye on the renal cell of freshwater fish Channa punctata. Sub-acute and chronic exposure to Nigrocine Black produced significant renal cellular degeneration in fish C. punctata. The 1/20th LC50 (96h) of the Nigrocine Black produced degenerating changes in the kidney tissues of fish. Renal corpuscles were revealing swollen and contained some degenerated RBC and thmbrocytes, which appeared to be cellular debris in Bowman space, due to the rapid destruction of the nuclei of erythrocytes. The liberated hemoglobin and other cytoplasmic materials were also observed, while 1/20th of LC50 (96hr) of Nigrosine Black produced renal degeneration in Bowman’s capsule and swelling in the glomerulus in the intoxicated fish along with renal tubules atrophy, degeneration as compared to the control group of fish. The low dose of Nigrosine Black Toxicity in fish produced nephropathy, and nephrosis. While the high dose of Nigrosine black dye produces Glomerulonephritis in experimental fish. The kidney cells of experimental fish are damaged by both doses of Nigrosine Black. The physico-chemical property of aquarium water was kept constant during whole duration of experiment.
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