Modern world can‘t be imagined without plastics due to their vast applications in contemporary life. All the plastic products which are available in market are made up of Petrochemical based synthetic plastics. Such plastics are much difficult to biodegrade which results in its wise accumulation in environment and affects biogeochemical cycles enormously. However, Bioplastics made up of Polyhydroxybutyrate (PHB) provide a better alternative to life threatening petrochemical plastics. PHB is a natural biopolymer produced by numerous bacteria by using different substrates and easy to extract. Thus on nature’s demand the author was performed an experiment with the aim isolate PHB producing bacteria form natural environment and optimized the PHB producing capability of selected isolates. Among twenty two bacterial isolates, five isolates were showed prominent PHB production at 37°C in presence of glucose and sucrose as a carbon source while peptone a nitrogen source in medium with pH 7.0. The isolates which showed more than 50% PHB production were identified as Bacillus licheniformis and Bacillus cereus. PHB polymer production by isolate was confirmed by UV spectrophotometer analysis.
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