Using a spectrophotofluorometric technic hydrogen peroxide and ultraviolet irradiation ( 1) Duggan and Udenfriend have shown that on these fluorescent curves. Initial studies esthe Vit. BG group could be detected in concen-tablished optimal conditions for fluorescence trations as low as 0.001 M/ml( 2 ) -We conof these compounds to be in 0.1 molar phosfirmed these observations, and extended the phate buffer of a p~ 6.75. The ideal conuse of this technic to demonstrate effects of centration for studying activity was found in *This work supported in part by grant from Hoff-the range of a 1 micromole/l- Table I outlines the fluorescence of a 1 micromolar stand-.man LaRo&e Labs. by guest on July 25, 2015
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